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<a href=""> | <a href=""> | ||
<div> | <div> | ||
− | <h> | + | <h>Preparation</h> |
<ol>1. Thaw competent cells rapidly by immersing frozen tubes in a 37°C water bath</ol> | <ol>1. Thaw competent cells rapidly by immersing frozen tubes in a 37°C water bath</ol> | ||
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<p align="center"><img src="https://static.igem.org/mediawiki/igem.org/d/d3/Transfo_pic_1.png" width="600px"/></p> | <p align="center"><img src="https://static.igem.org/mediawiki/igem.org/d/d3/Transfo_pic_1.png" width="600px"/></p> | ||
− | </div> | + | </div></a> |
− | <div> | + | <a href=""><div> |
<h>Digestion</h> | <h>Digestion</h> | ||
<ol>1. Select restriction enzymes to digest your plasmid. Determine an appropriate reaction buffer by reading the instructions for your enzyme. | <ol>1. Select restriction enzymes to digest your plasmid. Determine an appropriate reaction buffer by reading the instructions for your enzyme. | ||
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<ol>4. To visualize the results of your digest, conduct gel electrophoresis</ol> | <ol>4. To visualize the results of your digest, conduct gel electrophoresis</ol> | ||
− | </div> | + | </div></a> |
− | <div> | + | <a href=""><div> |
<h>Vector Preparation</h> | <h>Vector Preparation</h> | ||
<p>Combine the following in a PCR or Eppendorf tube:</p> | <p>Combine the following in a PCR or Eppendorf tube:</p> |
Revision as of 14:35, 29 July 2016