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<ol>8. Aliquot the competent cell (0.5 ml) in sterile tubes, freeze rapidly in liquid nitrogen or a dry-iced/ethanol bath or ice/isopropanol bath and store -70.</ol> | <ol>8. Aliquot the competent cell (0.5 ml) in sterile tubes, freeze rapidly in liquid nitrogen or a dry-iced/ethanol bath or ice/isopropanol bath and store -70.</ol> | ||
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<ol>4. Dilute the transformed cells as appropriate in T base containing 0.5% glucose and plate immediately onto selective media. </ol> | <ol>4. Dilute the transformed cells as appropriate in T base containing 0.5% glucose and plate immediately onto selective media. </ol> | ||
− | <p align="center"><img src="https://static.igem.org/mediawiki/igem.org/d/d3/Transfo_pic_1.png" width=" | + | <p align="center"><img src="https://static.igem.org/mediawiki/igem.org/d/d3/Transfo_pic_1.png" width="400px"/></p> |
</div></a> | </div></a> | ||
<a href=""><div> | <a href=""><div> |
Revision as of 14:36, 29 July 2016