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<ol>1. Select restriction enzymes to digest your plasmid. Determine an appropriate reaction buffer by reading the instructions for your enzyme. | <ol>1. Select restriction enzymes to digest your plasmid. Determine an appropriate reaction buffer by reading the instructions for your enzyme. | ||
In a 1.5mL tube combine the following: | In a 1.5mL tube combine the following: | ||
− | < | + | <li> - DNA </li> |
− | < | + | <li>- Restriction Enzyme(s)</li> |
− | < | + | <li>- Buffer </li> |
− | < | + | <li>- dH2O up to total volume</li> |
<p>Mix gently by pipetting. </p></ol> | <p>Mix gently by pipetting. </p></ol> | ||
Revision as of 14:51, 29 July 2016