Line 444: | Line 444: | ||
<h>Vector Preparation</h> | <h>Vector Preparation</h> | ||
<p>Combine the following in a PCR or Eppendorf tube:</p> | <p>Combine the following in a PCR or Eppendorf tube:</p> | ||
− | < | + | <ol>- 25ng Vector DNA</ol> |
<ul>- 75ng Insert DNA</ul> | <ul>- 75ng Insert DNA</ul> | ||
<ul>- Ligase Buffer (1μL/10μL reaction for 10X buffer, and 2μL/10μL reaction for 5X buffer)</ul> | <ul>- Ligase Buffer (1μL/10μL reaction for 10X buffer, and 2μL/10μL reaction for 5X buffer)</ul> |
Revision as of 14:52, 29 July 2016