Line 429: | Line 429: | ||
<ol>1. Select restriction enzymes to digest your plasmid. Determine an appropriate reaction buffer by reading the instructions for your enzyme. | <ol>1. Select restriction enzymes to digest your plasmid. Determine an appropriate reaction buffer by reading the instructions for your enzyme. | ||
In a 1.5mL tube combine the following: | In a 1.5mL tube combine the following: | ||
− | < | + | <ol> - DNA </ol> |
<li>- Restriction Enzyme(s)</li> | <li>- Restriction Enzyme(s)</li> | ||
<li>- Buffer </li> | <li>- Buffer </li> |
Revision as of 14:52, 29 July 2016