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<a href=""><div> | <a href=""><div> | ||
<h>Digestion</h> | <h>Digestion</h> | ||
− | < | + | <ul>1. Select restriction enzymes to digest your plasmid. Determine an appropriate reaction buffer by reading the instructions for your enzyme. |
− | In a 1.5mL tube combine the following:</ | + | In a 1.5mL tube combine the following:</ul> |
<ol> - DNA </ol> | <ol> - DNA </ol> | ||
<ol>- Restriction Enzyme(s)</ol> | <ol>- Restriction Enzyme(s)</ol> |
Revision as of 14:55, 29 July 2016