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<a href=""><div> | <a href=""><div> | ||
<h>Digestion</h> | <h>Digestion</h> | ||
− | < | + | <ol><li>1. Select restriction enzymes to digest your plasmid. Determine an appropriate reaction buffer by reading the instructions for your enzyme. </li> |
− | In a 1.5mL tube combine the following:</ | + | <p>In a 1.5mL tube combine the following:</p> |
− | < | + | <ul><li> - DNA </li> |
− | < | + | <li>- Restriction Enzyme(s)</li> |
− | < | + | <li>- Buffer </li> |
− | < | + | <li>- dH2O up to total volume</li> </ul> |
<p>Mix gently by pipetting. </p> | <p>Mix gently by pipetting. </p> | ||
Revision as of 14:57, 29 July 2016