Difference between revisions of "Team:Leiden/Biobricks"

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         Deinococcus radiodurans is an extremely radiation-resistant bacterium: while about 10 Gy (absorbed radiation dose, Gray) can kill most vertebrates, D. radiodurans can withstand up to 12,000 Gy. Current literature supports this finding with D. radiodurans’ unique genetic makeup which allows it to better handle radiation exposure. The two main effects of radiation exposure to bacterial cells are DNA damage and the creation of toxic superoxide species (Daly 2009). Two DNA damage prevention and repair proteins in D. radiodurans have been shown to outperform analogs in less radiation-tolerant bacteria. By binding to DNA, the protein DpsMP1 (DNA-binding proteins from starved cells) protects it from the reactive superoxide species formed by ionizing radiation (Slade and Miroslav 2011).
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         Deinococcus radiodurans is an extremely radiation-resistant bacterium: while about 10 Gy (absorbed radiation dose, Gray) can kill most vertebrates, D. radiodurans can withstand up to 12,000 Gy. Current literature supports this finding with D. radioduransâ&euro;&trade; unique genetic makeup which allows it to better handle radiation exposure. The two main effects of radiation exposure to bacterial cells are DNA damage and the creation of toxic superoxide species (Daly 2009). Two DNA damage prevention and repair proteins in D. radiodurans have been shown to outperform analogs in less radiation-tolerant bacteria. By binding to DNA, the protein DpsMP1 (DNA-binding proteins from starved cells) protects it from the reactive superoxide species formed by ionizing radiation (Slade and Miroslav 2011).
 
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Revision as of 17:24, 8 August 2016

iGEM Leiden Wiki / Biobricks

Biobricks

Biobricks

Name Web Description Length Type
K258005 http://parts.igem.org/Part:BBa_K258005 The promoter was maximally induced under microaerobic conditions (dissolved oxygen levels of less than 2% air saturation). Transcriptional activity decreased substantially under anaerobic conditions, suggesting the presence of a regulatory mechanism that is maximally induced under hypoxic but not completely anaerobic conditions in E.coli. Primer extension analysis was used to identify the existence of two overlapping promoterswithin a 150-base-pair region upstream of the structural VHb gene. The oxygen-dependent activity of both promoters was qualitatively similar, suggesting the existence of a common mechanism by which available oxygen concentrations influence expression from the two promoters. 137 bp Oxygen
K376003 http://parts.igem.org/Part:BBa_K376003 A composite promoter composed of FNR binding sites DcuC spacer region and the J23113 constitutive promoter. Activates transcription under micro-aerobic conditions. 83 bp Oxygen
K776021 http://parts.igem.org/Part:BBa_K776021 Complete system (dependent promoter oxygen concentration) Prom J23104 + RBS + PrrA + RBS + PrrB + RBS + PrrC + B0014 + Prom PrrA + RBS + GFP + B0014 0 bp ?? Oxygen
K847002 http://parts.igem.org/Part:BBa_K847002 Deinococcus radiodurans is an extremely radiation-resistant bacterium: while about 10 Gy (absorbed radiation dose, Gray) can kill most vertebrates, D. radiodurans can withstand up to 12,000 Gy. Current literature supports this finding with D. radiodurans’ unique genetic makeup which allows it to better handle radiation exposure. The two main effects of radiation exposure to bacterial cells are DNA damage and the creation of toxic superoxide species (Daly 2009). Two DNA damage prevention and repair proteins in D. radiodurans have been shown to outperform analogs in less radiation-tolerant bacteria. By binding to DNA, the protein DpsMP1 (DNA-binding proteins from starved cells) protects it from the reactive superoxide species formed by ionizing radiation (Slade and Miroslav 2011). 762 bp Radiation
R0011 http://parts.igem.org/Part:BBa_R0011 Promoter (lacI regulated, lambda pL hybrid). Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be: (1) repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0012) ([LUTZ97]); (2) induced by IPTG in E.Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range. 55 bp Promotor
K608008 http://parts.igem.org/Part:BBa_K608008 Constitutive strong promoter with medium RBS and GFP.Strong promoter from the constitutive promoter family combined with medium RBS (PR2) for strong gene expression. To quantify the gene expression, GFP was tagged to the promoter and RBS domain. 782 bp Promotor
K608014 http://parts.igem.org/Part:BBa_K608014 Strong promoter and medium RBS with RFP. This part consists of a strong promoter with medium RBS (PR2) and tagged with RFP to quantify the expression. 768 bp Promotor
K808000 http://parts.igem.org/Part:BBa_K808000 araC-Pbad - Arabinose inducible regulatory promoter/repressor unit.This part contains the promoter as well as the coding sequence for the repressor AraC which is transcribed in the opposite direction. (upstream) By binding to L(+)-arabinose, AraC changes its conformation. This causes the protein to diffuses from the DNA thereby inducing transcription. 1209 bp Promotor
K608010 http://parts.igem.org/Part:BBa_K608010 Medium promoter with strong RBS and GFP. Medium promoter from the constitutive promoter family combined with a strong RBS (PR4) and tagged with GFP to quantify the gene expression. 781 bp Promotor
K608012 http://parts.igem.org/Part:BBa_K608012 Medium promoter with weak RBS and GFP. Medium promoter from the constitutive promoter family combined with a weak RBS (PR6) and tagged with GFP to quantify the gene expression. 783 bp Promotor

Backbones

Name Web Description Length Resistance
pSB1A3 http://parts.igem.org/Part:pSB1A3 High copy BioBrick assembly plasmid 2155 Ampicillin
K376003 http://parts.igem.org/Part:pSB1C3 High copy BioBrick assembly plasmid 2070 Chloramphenicol
K776021 http://parts.igem.org/Part:pSB1T3 High copy BioBrick assembly plasmid 2461 tetracycline
K847002 http://parts.igem.org/Part:pSB1K3 High copy BioBrick assembly plasmid 2204 Kanamycin