Difference between revisions of "Team:Paris Bettencourt/Notebook/Indigo"

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<h1 class="red">Week 4th - 10th July</h1>
 
<h1 class="red">Week 4th - 10th July</h1>
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<p class=”input”> We received the <i>E. coli</i> BL21 and BL21(DE3) - <b>same background, but BL21 does not express the T7 polimerase and BL21(DE3) does.</b><br>
 +
<u>Highlights</u><br>
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Transformation efficiency: 1–5 x 107 cfu/μg pUC19 DNA<br>
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Routine non-T7 expression<br>
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Deficient in proteases Lon and OmpT<br>
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Resistant to phage T1 (<i>fhuA2</i>)<br>
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B Strain<br>
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Free of animal products<br>
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<u>Genotype</u><br>
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<i>fhuA2 [lon] ompT gal [dcm] ΔhsdS</i></p>
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<h1 class="red">Week 11th - 17th July</h1>
 
<h1 class="red">Week 11th - 17th July</h1>

Revision as of 18:32, 10 August 2016


Week 27th June - 3rd July

Week 4th - 10th July

We received the E. coli BL21 and BL21(DE3) - same background, but BL21 does not express the T7 polimerase and BL21(DE3) does.
Highlights
Transformation efficiency: 1–5 x 107 cfu/μg pUC19 DNA
Routine non-T7 expression
Deficient in proteases Lon and OmpT
Resistant to phage T1 (fhuA2)
B Strain
Free of animal products
Genotype
fhuA2 [lon] ompT gal [dcm] ΔhsdS

Week 11th - 17th July

Week 18th -24th July

Week 25th -31th July


Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
+33 1 44 41 25 22/25
igem2016parisbettencourt@gmail.com
2016.igem.org