Difference between revisions of "Team:XMU-China"

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{{XMU-China}}
 
{{XMU-China}}
 
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<h2> Brief Introduction </h2>
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<p>The bacteria is one of the pathogen of humans. For many years, overusing of antibiotic contributes to antibiotic resistance of many bacteria. At present, drug-resistant bacteria are becoming a huge threaten to human's life health, the development of new antibiotics can't catch its demanding. Focusing on this, our team uses the way of synthetic biology to detect and kill the drug-resistant bacteria.</p>
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<p>On gram-positive bacterium, we choose the <cite>MRSA</cite> to study. We make use of the quorum-sensing system of gram-positive bacterium and a genetic switch based on <cite>cI</cite> and <cite>lacI</cite> genes, then we design a genetic circuit to detect <cite>MRSA</cite> and then kill it by producing a toxin, and we put our genetic circuit into the <cite>E.coli</cite> to produce a new <cite>E.coli</cite> that can detect <cite>MRSA</cite> specifically and then kill it. In our experiment, we use <cite>MSSA</cite> to study because <cite>MRSA</cite> is a second-level pathogenic bacteria. Now, our present work is connecting the plasmids.</p>
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<p>In terms of gram-negative bacterium，<cite>E. coli</cite> was selected and the engineering bacteria was designed to resist antibiotic resistance <cite>E. coli</cite>. After the engineering bacteria detects the group sense signal molecules of pathogen, siRNA which will silence the gene allowing a bacterium to be resistant to antibiotics is released. Meanwhile, special enzyme is emitted from the engineering bacteria to destroy the biofilm. At this stage we designed and synthesized siRNA, and the next step is to carry out the in vitro transcription experiment of siRNA.</p>
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<h2> Welcome to iGEM 2016! </h2>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h5>Before you start: </h5>
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<p> Please read the following pages:</p>
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<ul class="sui-menu">
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    <li style="background-color: #E76B56;"><a style="color: #fff;" href="">Home</a></li>
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    <li style="background-color: #F4A460;"><a style="color: #fff;" href="">Project</a></li>
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    <li style="background-color: #FFB436;"><a style="color: #fff;" href="">Interlab</a></li>
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    <li style="background-color: #A2CD5A;"><a style="color: #fff;" href="">Safety</a></li>
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    <li style="background-color: #8FBC8F;"><a style="color: #fff;" href="">Note</a></li>
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    <li style="background-color: #8DB6CD;"><a style="color: #fff;" href="">Human Practice</a></li>
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    <li style="background-color: #363D68;"><a style="color: #fff;" href="">Team</a></li>
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  </ul>
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<div id="sidebar">
 
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<li> <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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<a href="#hea">Project Description</a>
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<li> <a href="https://2016.igem.org/Resources/Template_Documentation"> Template Documentation </a></li>
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    <ul>
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    <li><a href="#Gen">MRSA Killer</a></li>
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      <li><a href="#For">Formaldehyde Eliminator</a></li>
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        </ul>
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</li>
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<li onmouseover="showsubmenu(this)" onmouseout="hidesubmenu(this)"><a href="">Results</a>
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<ul>
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    <li>Description</li>
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      <li>2</li>
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      <li>3</li>
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        <li>4</li>
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    <li>1</li>
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      <li>2</li>
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      <li>3</li>
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        <li>4</li>
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        </ul>
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</li>
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<li onmouseover="showsubmenu(this)" onmouseout="hidesubmenu(this)"><a href="">Future Work</a>
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<ul>
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    <li>1</li>
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      <li>2</li>
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      <li>3</li>
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        <li>4</li>
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        </ul>
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<div class="main">
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<div class="content" id="content">
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<div class="highlight">
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<h1 id="hea">Project Description</h1>
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<h5> Styling your wiki </h5>
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<div id="Gen" class="item">
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<h2>MRSA Killer</h2>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<p>The bacteria is one of the pathogen of humans. For many years, overusing of antibiotic contributes to antibiotic resistance of many bacteria. At present, drug-resistant bacteria are becoming a huge threaten to human's life health, the development of new antibiotics can't catch its demanding. Focusing on this, our team uses the way of synthetic biology to detect and kill the drug-resistant bacteria.</p>
 +
<br/>
 +
<p>On gram-positive bacterium, we choose the MRSA to study. We make use of the quorum-sensing system of gram-positive bacterium and a genetic switch based on cI and lacI genes, then we design a genetic circuit to detect MRSA and then kill it by producing a toxin, and we put our genetic circuit into the E.coli to produce a new E.coli that can detect MRSA specifically and then kill it. In our experiment, we use MSSA to study because MRSA is a second-level pathogenic bacteria. Now, our present work is connecting the plasmids.</p>
 +
<br/>
 +
<p>In terms of gram-negative bacterium，E. coli was selected and the engineering bacteria was designed to resist antibiotic resistance E. coli. After the engineering bacteria detects the group sense signal molecules of pathogen, siRNA which will silence the gene allowing a bacterium to be resistant to antibiotics is released. Meanwhile, special enzyme is emitted from the engineering bacteria to destroy the biofilm. At this stage we designed and synthesized siRNA, and the next step is to carry out the in vitro transcription experiment of siRNA.</p>
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<br/>
 
</div>
 
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<hr/>
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<div id="For" class="item">
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<h2>Formaldehyde Eliminator</h2>
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<p>This year the project of XMU-China is Formaldehyde Eliminator.Formaldehyde is an important precursor to many other materials and chemical compounds. It is mainly used in the production of industrial resins, e.g., for particle board and coatings.</p>
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<br/>
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<p>In view of its widespread use, toxicity, and volatility, formaldehyde poses a significant danger to human health.[8] In 2011, the US National Toxicology Program described formaldehyde as "known to be a human carcinogen"We focused on the formaldhyde sensing and recycling. In this project, we conducted three circuits to get access to our target which is about detection and transformation of formaldehyde. With the Sensor, we can tell, from the green fluorescence and the forming of patterns, whether there is formaldehyde or not and whether the formaldehyde is eliminated completely. Recycle circuit could change formaldehyde into methanol and carbon dioxide through multiple metabolism with the help of specific enzymes. The Controller circuit activates Recycle circuit, releasing the enzymes in the presence of formaldehyde, in which way it enhances the function of the Recycle System. With the cooperation of all these three systems, we can detect and transform formaldehyde by a biosynthetic way.</p> 
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<h5> Wiki template information </h5>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<h5> Editing your wiki </h5>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<h5>Tips</h5>
 
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 
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<ul>
 
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
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<li>Be clear about what you are doing and how you plan to do this.</li>
 
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
 
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<li>Have lots of fun! </li>
 
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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UPLOAD FILES
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Revision as of 15:28, 12 August 2016



Project Description

MRSA Killer

The bacteria is one of the pathogen of humans. For many years, overusing of antibiotic contributes to antibiotic resistance of many bacteria. At present, drug-resistant bacteria are becoming a huge threaten to human's life health, the development of new antibiotics can't catch its demanding. Focusing on this, our team uses the way of synthetic biology to detect and kill the drug-resistant bacteria.


On gram-positive bacterium, we choose the MRSA to study. We make use of the quorum-sensing system of gram-positive bacterium and a genetic switch based on cI and lacI genes, then we design a genetic circuit to detect MRSA and then kill it by producing a toxin, and we put our genetic circuit into the E.coli to produce a new E.coli that can detect MRSA specifically and then kill it. In our experiment, we use MSSA to study because MRSA is a second-level pathogenic bacteria. Now, our present work is connecting the plasmids.


In terms of gram-negative bacterium，E. coli was selected and the engineering bacteria was designed to resist antibiotic resistance E. coli. After the engineering bacteria detects the group sense signal molecules of pathogen, siRNA which will silence the gene allowing a bacterium to be resistant to antibiotics is released. Meanwhile, special enzyme is emitted from the engineering bacteria to destroy the biofilm. At this stage we designed and synthesized siRNA, and the next step is to carry out the in vitro transcription experiment of siRNA.



Formaldehyde Eliminator

This year the project of XMU-China is Formaldehyde Eliminator.Formaldehyde is an important precursor to many other materials and chemical compounds. It is mainly used in the production of industrial resins, e.g., for particle board and coatings.


In view of its widespread use, toxicity, and volatility, formaldehyde poses a significant danger to human health.[8] In 2011, the US National Toxicology Program described formaldehyde as "known to be a human carcinogen"We focused on the formaldhyde sensing and recycling. In this project, we conducted three circuits to get access to our target which is about detection and transformation of formaldehyde. With the Sensor, we can tell, from the green fluorescence and the forming of patterns, whether there is formaldehyde or not and whether the formaldehyde is eliminated completely. Recycle circuit could change formaldehyde into methanol and carbon dioxide through multiple metabolism with the help of specific enzymes. The Controller circuit activates Recycle circuit, releasing the enzymes in the presence of formaldehyde, in which way it enhances the function of the Recycle System. With the cooperation of all these three systems, we can detect and transform formaldehyde by a biosynthetic way.