Difference between revisions of "Team:BGIC China/protocols/dna-electrophoresis"
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| − | + | </style><!-- react-empty: 12 --><!-- react-empty: 13 --><ul class="slides s-page-1" data-reactid="14"><li class="slide" id="section-f_842c2e69-ecae-4a76-86ee-417c2dfdf6eb" data-reactid="15"><div class="waypoint" data-reactid="16"></div><a class="section-anchor" data-reactid="17"></a><div class="s-section s-text-section" data-reactid="18"><div class="container" data-reactid="19"><div class="clearfix" data-reactid="20"><div class="columns sixteen" data-reactid="21"><div class="s-title-group s-only-title-group" data-reactid="22"><div data-reactid="23"><div class="s-title " data-reactid="24"><div class="s-component s-text" data-reactid="25"><h1 class="s-component-content s-font-title" data-reactid="26"><p>1.5%琼脂凝胶配制</p></h1></div></div><div class="s-subtitle" data-reactid="27"><div class="s-component s-text" data-reactid="28"><h4 class="s-component-content s-font-heading" data-reactid="29"><p style="text-align:left">小胶30ml TAE、中胶60ml TAE、大胶120ml TAE</p><p style="text-align:left">加入1.5%琼脂糖粉</p><p style="text-align:left">放入微波炉加热至沸腾</p><p style="text-align:left">等待降温到60℃后加入10000x染料摇晃混匀</p><p style="text-align:left">将胶倒入胶槽中</p></h4></div></div></div></div></div></div><!-- react-text: 30 --> <!-- /react-text --><div class=" s-repeatable" data-reactid="31"><div data-reactid="32"></div></div><!-- react-text: 33 --> <!-- /react-text --></div></div></li><li class="slide" id="section-f_46b864b0-cf37-4095-9abd-fcb6a73a1a20" data-reactid="34"><div class="waypoint" data-reactid="35"></div><a class="section-anchor" data-reactid="36"></a><div class="s-section s-text-section" data-reactid="37"><div class="container" data-reactid="38"><div class="clearfix" data-reactid="39"><div class="columns sixteen" data-reactid="40"><div class="s-title-group s-only-title-group" data-reactid="41"><div data-reactid="42"><div class="s-title " data-reactid="43"><div class="s-component s-text" data-reactid="44"><h2 class="s-component-content s-font-title" data-reactid="45"><p>电泳程序</p></h2></div></div><div class="s-subtitle" data-reactid="46"><div class="s-component s-text" data-reactid="47"><h4 class="s-component-content s-font-heading" data-reactid="48"><p style="text-align: left;">DNA溶液中先加入10x loading buffer</p><p style="text-align: left;">Vortex混匀</p><p style="text-align: left;">点样和加入DNA ladder</p><p style="text-align: left;">电压120v</p><p style="text-align: left;">电流300mA</p><p style="text-align: left;">时间30min</p></h4></div></div></div></div></div></div><!-- react-text: 49 --> <!-- /react-text --><div class=" s-repeatable" data-reactid="50"><div data-reactid="51"></div></div><!-- react-text: 52 --> <!-- /react-text --></div></div></li><li class="slide" id="section-f_e63ff7e3-0034-44ac-86b1-585358cac151" data-reactid="53"><div class="waypoint" data-reactid="54"></div><a class="section-anchor" data-reactid="55"></a><div class="s-columns-section s-section" data-reactid="56"><div class="container" data-reactid="57"><div class="columns sixteen" data-reactid="58"><div class="s-title-group " data-reactid="59"><div data-reactid="60"><div class="s-title " data-reactid="61"><div class="s-component s-text" data-reactid="62"><h2 class="s-component-content s-font-title" data-reactid="63"><p>耗材来源及信息</p></h2></div></div></div></div></div><!-- react-text: 64 --> <!-- /react-text --><div class="s-mh s-repeatable" data-reactid="65"><div data-reactid="66"><div data-sorting-index="0" class="four columns no-float s-repeatable-item" data-reactid="67"><div class="s-item-media-group s-mhi" data-reactid="68"><div class="" data-reactid="69"><div class="s-component s-media " data-reactid="70"><span data-reactid="71"></span></div></div></div><div class="s-item-text-group " data-reactid="72"><div class="s-item-subtitle" data-reactid="73"><div class="s-component s-text" data-reactid="74"><h6 class="s-component-content s-font-body" data-reactid="75"><p>TAE缓冲液母液<strong></strong></p></h6></div></div><div class="s-item-text" data-reactid="76"><div class="s-component s-text" data-reactid="77"><div class="s-component-content s-font-body" data-reactid="78"><p style="text-align: left;">生产厂家：</p><p style="text-align: left;">型号：</p><p style="text-align: left;">内含耗材：</p></div></div></div></div></div><div data-sorting-index="1" class="four columns no-float s-repeatable-item" data-reactid="79"><div class="s-item-media-group s-mhi" data-reactid="80"><div class="" data-reactid="81"><div class="s-component s-media " data-reactid="82"><span data-reactid="83"></span></div></div></div><div class="s-item-text-group " data-reactid="84"><div class="s-item-subtitle" data-reactid="85"><div class="s-component s-text" data-reactid="86"><h6 class="s-component-content s-font-body" data-reactid="87"><p>琼脂糖粉<strong></strong></p></h6></div></div><div class="s-item-text" data-reactid="88"><div class="s-component s-text" data-reactid="89"><div class="s-component-content s-font-body" data-reactid="90"><p style="text-align: left;">生产厂家：</p><p style="text-align: left;">型号：</p><p style="text-align: left;">内含耗材：</p></div></div></div></div></div><div data-sorting-index="2" class="four columns no-float s-repeatable-item" data-reactid="91"><div class="s-item-media-group s-mhi" data-reactid="92"><div class="" data-reactid="93"><div class="s-component s-media " data-reactid="94"><span data-reactid="95"></span></div></div></div><div class="s-item-text-group " data-reactid="96"><div class="s-item-subtitle" data-reactid="97"><div class="s-component s-text" data-reactid="98"><h6 class="s-component-content s-font-body" data-reactid="99"><p><strong>DNA染料</strong></p></h6></div></div><div class="s-item-text" data-reactid="100"><div class="s-component s-text" data-reactid="101"><div class="s-component-content s-font-body" data-reactid="102"><p style="text-align:left">生产厂家：</p><p style="text-align:left">型号：</p><p style="text-align:left">内含耗材：</p></div></div></div></div></div><div data-sorting-index="3" class="four columns no-float s-repeatable-item" data-reactid="103"><div class="s-item-media-group s-mhi" data-reactid="104"><div class="" data-reactid="105"><div class="s-component s-media " data-reactid="106"><span data-reactid="107"></span></div></div></div><div class="s-item-text-group " data-reactid="108"><div class="s-item-subtitle" data-reactid="109"><div class="s-component s-text" data-reactid="110"><h6 class="s-component-content s-font-body" data-reactid="111"><p><strong>10x loading buffer</strong></p></h6></div></div><div class="s-item-text" data-reactid="112"><div class="s-component s-text" data-reactid="113"><div class="s-component-content s-font-body" data-reactid="114"><p style="text-align:left">生产厂家：</p><p style="text-align:left">型号：</p><p style="text-align:left">内含耗材：</p></div></div></div></div></div><div data-sorting-index="4" class="four columns no-float s-repeatable-item" data-reactid="115"><div class="s-item-media-group s-mhi" data-reactid="116"><div class="" data-reactid="117"><div 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