Difference between revisions of "Team:BGIC China/protocols"

Latest revision as of 15:48, 19 August 2016

Ellz's Site

Protocols
从下面找到你想用的Protocol并点击

Q5 PCR
Q5 PCR的配方以及程序
DNA电泳
琼脂凝胶的配方以及电泳程序
胶回收
琼脂凝胶DNA回收步骤
酶切
酶切体系以及程序
纯化
线性DNA过柱纯化
T4 连接
使用T4连接酶进行DNA连接
转化
将质粒转化进感受态
提质粒
少壮不努力的结果
引物磷酸化
未磷酸化的引物合成DNA片段
LB培养基
配制固体和液体LB培养基
M9基础培养基
配制无碳源的M9基础培养基
菌落PCR
菌落PCR配方及程序
S30系统反应
S30无细胞体系的反应步骤
蛋白电泳
蛋白胶配方及蛋白电泳程序

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-{{BGIC_China_iot}}
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+.s-custom-colors .s-section.s-info-section.s-bg-dark-text .s-info-box .s-item-title a,
-<p><font color="#1C2833" size=5>常用Protocols</font></p>
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+.s-custom-colors .s-section.s-info-section.s-bg-dark-text .s-info-box .s-item-title a:hover,
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+.s-custom-colors .s-section.s-info-section.s-bg-light-text .s-info-box .s-item-text a:hover,
+.s-custom-colors .s-section.s-info-section.s-bg-dark-text .s-info-box .s-item-text a:hover {
+  color: #777dc8;
+  border-bottom-color: #777dc8;
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-<tr>
+.s-custom-colors .s-text-color-custom1{ color: #777dc8; }
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+</style><!-- react-empty: 12 --><!-- react-empty: 13 --><ul class="slides s-page-1" data-reactid="14"><li class="slide" id="section-f_23391e54-383f-44d8-99d4-dfe469d501d5" data-reactid="15"><div class="waypoint" data-reactid="16"></div><a class="section-anchor" data-reactid="17"></a><div class="lazyload s-no-bg s-info-section s-section" data-reactid="18"><div class="container" data-reactid="19"><div class="columns sixteen" data-reactid="20"><div class="s-title-group " data-reactid="21"><div data-reactid="22"><div class="s-title " data-reactid="23"><div class="s-component s-text" data-reactid="24"><h1 class="s-component-content s-font-title" data-reactid="25"><p>Protocols</p></h1></div></div><div class="s-subtitle" data-reactid="26"><div class="s-component s-text" data-reactid="27"><h4 class="s-component-content s-font-heading" data-reactid="28"><p>从下面找到你想用的Protocol并点击</p></h4></div></div></div></div></div><!-- react-text: 29 --> <!-- /react-text --><div class="s-mh s-repeatable" data-reactid="30"><div data-reactid="31"><div data-sorting-index="0" class="four columns no-float s-repeatable-item" data-reactid="32"><div class="s-item-text-group s-info-box s-mhi " data-reactid="33"><div class="s-item-title" data-reactid="34"><div class="s-component s-text" data-reactid="35"><h3 class="s-component-content s-font-heading" data-reactid="36"><p><a href="https://2016.igem.org/Team:BGIC_China/protocols/q5-pcr">Q5 PCR</a></p></h3></div></div><div class="s-item-text" data-reactid="37"><div class="s-component s-text" data-reactid="38"><div class="s-component-content s-font-body" data-reactid="39"><p style="font-size: 80%;">Q5 PCR的配方以及程序</p></div></div></div></div></div><div data-sorting-index="1" class="four columns no-float s-repeatable-item" data-reactid="40"><div class="s-item-text-group s-info-box s-mhi " data-reactid="41"><div class="s-item-title" data-reactid="42"><div class="s-component s-text" data-reactid="43"><h3 class="s-component-content s-font-heading" data-reactid="44"><p><a href="https://2016.igem.org/Team:BGIC_China/protocols/dna-electrophoresis">DNA电泳</a></p></h3></div></div><div class="s-item-text" data-reactid="45"><div class="s-component s-text" data-reactid="46"><div class="s-component-content s-font-body" data-reactid="47"><p style="font-size: 80%;">琼脂凝胶的配方以及电泳程序</p></div></div></div></div></div><div data-sorting-index="2" class="four columns no-float s-repeatable-item" data-reactid="48"><div class="s-item-text-group s-info-box s-mhi " data-reactid="49"><div class="s-item-title" data-reactid="50"><div class="s-component s-text" data-reactid="51"><h3 class="s-component-content s-font-heading" data-reactid="52"><p><a href="https://2016.igem.org/Team:BGIC_China/protocols/gel-recovery">胶回收</a></p></h3></div></div><div class="s-item-text" data-reactid="53"><div class="s-component s-text" data-reactid="54"><div class="s-component-content s-font-body" data-reactid="55"><p style="font-size: 80%;">琼脂凝胶DNA回收步骤</p></div></div></div></div></div><div data-sorting-index="3" class="four columns no-float s-repeatable-item" data-reactid="56"><div class="s-item-text-group s-info-box s-mhi " data-reactid="57"><div class="s-item-title" data-reactid="58"><div class="s-component s-text" data-reactid="59"><h3 class="s-component-content s-font-heading" data-reactid="60"><p><a href="https://2016.igem.org/Team:BGIC_China/protocols/restriction-digestion">酶切</a></p></h3></div></div><div class="s-item-text" data-reactid="61"><div class="s-component s-text" data-reactid="62"><div class="s-component-content s-font-body" data-reactid="63"><p style="font-size: 80%;">酶切体系以及程序</p></div></div></div></div></div><div data-sorting-index="4" class="four columns no-float s-repeatable-item" data-reactid="64"><div class="s-item-text-group s-info-box s-mhi " data-reactid="65"><div class="s-item-title" data-reactid="66"><div class="s-component s-text" data-reactid="67"><h3 class="s-component-content s-font-heading" data-reactid="68"><p><a href="https://2016.igem.org/Team:BGIC_China/protocols/dna-purification">纯化</a></p></h3></div></div><div class="s-item-text" data-reactid="69"><div class="s-component s-text" data-reactid="70"><div class="s-component-content s-font-body" data-reactid="71"><p style="font-size: 80%;">线性DNA过柱纯化</p></div></div></div></div></div><div data-sorting-index="5" class="four columns no-float s-repeatable-item" data-reactid="72"><div class="s-item-text-group s-info-box s-mhi " data-reactid="73"><div class="s-item-title" data-reactid="74"><div class="s-component s-text" data-reactid="75"><h3 class="s-component-content s-font-heading" data-reactid="76"><p><a href="https://2016.igem.org/Team:BGIC_China/protocols/T4-ligation">T4 连接</a></p></h3></div></div><div class="s-item-text" data-reactid="77"><div class="s-component s-text" data-reactid="78"><div class="s-component-content s-font-body" data-reactid="79"><p style="font-size: 80%;">使用T4连接酶进行DNA连接</p></div></div></div></div></div><div data-sorting-index="6" class="four columns no-float s-repeatable-item" data-reactid="80"><div class="s-item-text-group s-info-box s-mhi " data-reactid="81"><div class="s-item-title" data-reactid="82"><div class="s-component s-text" data-reactid="83"><h3 class="s-component-content s-font-heading" data-reactid="84"><p><a href="https://2016.igem.org/Team:BGIC_China/protocols/transformation">转化</a></p></h3></div></div><div class="s-item-text" data-reactid="85"><div class="s-component s-text" data-reactid="86"><div class="s-component-content s-font-body" data-reactid="87"><p style="font-size: 80%;">将质粒转化进感受态</p></div></div></div></div></div><div data-sorting-index="7" class="four columns no-float s-repeatable-item" data-reactid="88"><div class="s-item-text-group s-info-box s-mhi " data-reactid="89"><div class="s-item-title" data-reactid="90"><div class="s-component s-text" data-reactid="91"><h3 class="s-component-content s-font-heading" data-reactid="92"><p><a href="https://2016.igem.org/Team:BGIC_China/protocols/plasmid-extraction">提质粒</a></p></h3></div></div><div class="s-item-text" data-reactid="93"><div class="s-component s-text" data-reactid="94"><div class="s-component-content s-font-body" data-reactid="95"><p style="font-size: 80%;">少壮不努力的结果</p></div></div></div></div></div><div data-sorting-index="8" class="four columns no-float s-repeatable-item" data-reactid="96"><div class="s-item-text-group s-info-box s-mhi " data-reactid="97"><div class="s-item-title" data-reactid="98"><div class="s-component s-text" data-reactid="99"><h3 class="s-component-content s-font-heading" data-reactid="100"><p><a href="https://2016.igem.org/Team:BGIC_China/protocols/primers-phosphorylation">引物磷酸化</a></p></h3></div></div><div class="s-item-text" data-reactid="101"><div class="s-component s-text" data-reactid="102"><div class="s-component-content s-font-body" data-reactid="103"><p style="font-size: 80%;">未磷酸化的引物合成DNA片段</p></div></div></div></div></div><div data-sorting-index="9" class="four columns no-float s-repeatable-item" data-reactid="104"><div class="s-item-text-group s-info-box s-mhi " data-reactid="105"><div class="s-item-title" data-reactid="106"><div class="s-component s-text" data-reactid="107"><h3 class="s-component-content s-font-heading" data-reactid="108"><p><a href="https://2016.igem.org/Team:BGIC_China/protocols/LB-culture">LB培养基</a></p></h3></div></div><div class="s-item-text" data-reactid="109"><div class="s-component s-text" data-reactid="110"><div class="s-component-content s-font-body" data-reactid="111"><p style="font-size: 80%;">配制固体和液体LB培养基</p></div></div></div></div></div><div data-sorting-index="10" class="four columns no-float s-repeatable-item" data-reactid="112"><div class="s-item-text-group s-info-box s-mhi " data-reactid="113"><div class="s-item-title" data-reactid="114"><div class="s-component s-text" data-reactid="115"><h3 class="s-component-content s-font-heading" data-reactid="116"><p><a href="https://2016.igem.org/Team:BGIC_China/protocols/M9-culture">M9基础培养基</a></p></h3></div></div><div class="s-item-text" data-reactid="117"><div class="s-component s-text" data-reactid="118"><div class="s-component-content s-font-body" data-reactid="119"><p style="font-size: 80%;">配制无碳源的M9基础培养基</p></div></div></div></div></div><div data-sorting-index="11" class="four columns no-float s-repeatable-item" data-reactid="120"><div class="s-item-text-group s-info-box s-mhi " data-reactid="121"><div class="s-item-title" data-reactid="122"><div class="s-component s-text" data-reactid="123"><h3 class="s-component-content s-font-heading" data-reactid="124"><p><a href="https://2016.igem.org/Team:BGIC_China/protocols/colony-pcr">菌落PCR</a></p></h3></div></div><div class="s-item-text" data-reactid="125"><div class="s-component s-text" data-reactid="126"><div class="s-component-content s-font-body" data-reactid="127"><p style="font-size: 80%;">菌落PCR配方及程序</p></div></div></div></div></div><div data-sorting-index="12" class="four columns no-float s-repeatable-item" data-reactid="128"><div class="s-item-text-group s-info-box s-mhi " data-reactid="129"><div class="s-item-title" data-reactid="130"><div class="s-component s-text" data-reactid="131"><h3 class="s-component-content s-font-heading" data-reactid="132"><p><a href="https://2016.igem.org/Team:BGIC_China/protocols/S30-reaction">S30系统反应</a></p></h3></div></div><div class="s-item-text" data-reactid="133"><div class="s-component s-text" data-reactid="134"><div class="s-component-content s-font-body" data-reactid="135"><p style="font-size: 80%;">S30无细胞体系的反应步骤</p></div></div></div></div></div><div data-sorting-index="13" class="four columns no-float s-repeatable-item" data-reactid="136"><div class="s-item-text-group s-info-box s-mhi " data-reactid="137"><div class="s-item-title" data-reactid="138"><div class="s-component s-text" data-reactid="139"><h3 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