User contributions
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- 14:11, 19 August 2016 (diff | hist) . . (+26) . . Team:Paris Saclay/Notebook/August/10 (→Phusion PCR on the ligation products)
- 14:08, 19 August 2016 (diff | hist) . . (+5) . . Team:Paris Saclay/Notebook/August/10 (→PCR Clean-up with the NucleoSpin kit)
- 14:07, 19 August 2016 (diff | hist) . . (+1) . . Team:Paris Saclay/Notebook/August/9 (→Extraction of pPS16_001, pPS16_005, pPS16_006, pPS16_007 and pPS16_009)
- 14:06, 19 August 2016 (diff | hist) . . (+332) . . Team:Paris Saclay/Notebook/August/9 (→Visualization)
- 13:50, 19 August 2016 (diff | hist) . . (+32) . . Team:Paris Saclay/Notebook/August/9 (→Phusion PCR on pPS16_006 and pPS16_007)
- 13:09, 19 August 2016 (diff | hist) . . (-155) . . Team:Paris Saclay/Notebook/August/5 (→Friday 5st August)
- 12:58, 19 August 2016 (diff | hist) . . (0) . . Team:Paris Saclay/Notebook/August/4 (→Thursday 4st August)
- 12:56, 19 August 2016 (diff | hist) . . (0) . . Team:Paris Saclay/Notebook/August/3 (→Wednesday 3st August)
- 10:24, 19 August 2016 (diff | hist) . . (+51) . . Team:Paris Saclay/Notebook/August/19 (→Colony and Extraction product DreamTaq PCR)
- 10:20, 19 August 2016 (diff | hist) . . (+75) . . Team:Paris Saclay/Notebook/August/19 (→Colony and Extraction product DreamTaq PCR)
- 10:16, 19 August 2016 (diff | hist) . . (+112) . . Team:Paris Saclay/Notebook/August/19 (→Colony and Extraction product DreamTaq PCR)
- 09:56, 19 August 2016 (diff | hist) . . (+488) . . Team:Paris Saclay/Notebook/August/19 (→Friday 19th August)
- 09:48, 19 August 2016 (diff | hist) . . (+229) . . Team:Paris Saclay/Notebook/August/18 (→Visualization)
- 09:41, 19 August 2016 (diff | hist) . . (+591) . . Team:Paris Saclay/Notebook/August/18 (→DreamTaq PCR on DH5a colonies transformed on the 17/08/2016 with PJet containing 1.2, FRB, FKBP or sgRNA Nm gBlocks)
- 09:41, 19 August 2016 (diff | hist) . . (+528) . . N File:T--Paris Saclay--160818 gel3.jpeg (Gel Electrophoresis of PCR product Pjet-FRB and FKBP. Expected size: FRB : gBlock = 374bp + 118bp (number of bp between the two primers on Pjet) = 492. Clone 2 to 6 seem to have been transformed with Pjet containing the right insert. Clones 3, 4 and 5...) (current)
- 09:37, 19 August 2016 (diff | hist) . . (+366) . . Team:Paris Saclay/Notebook/August/18 (→DreamTaq PCR on DH5a colonies transformed on the 17/08/2016 with PJet containing 1.2, FRB, FKBP or sgRNA Nm gBlocks)
- 09:36, 19 August 2016 (diff | hist) . . (+331) . . N File:T--Paris Saclay--160818 gel2.jpeg (Gel Electrophoresis of PCR product Pjet-1.2. Expected size: gBlock = 960bp + 118bp (number of bp between the two primers on Pjet) = 1078. Clone 8 and 11 seem to have been transformed with Pjet containing the right insert. It will be extracted, checked...) (current)
- 09:30, 19 August 2016 (diff | hist) . . (+303) . . N File:T--Paris Saclay--160818 gel1.jpeg (Gel Electrophoresis of PCR product Pjet-sgRNA. Expected size: gBlock = 362bp + 118bp (number of bp between the two primers on Pjet) = 480. Clone 5 seems to have been transformed with Pjet containing the right insert.It will be extracted, checked and se...) (current)
- 09:29, 19 August 2016 (diff | hist) . . (+1) . . Team:Paris Saclay/Notebook/August/18 (→DreamTaq PCR on DH5a colonies transformed on the 17/08/2016 with PJet containing 1.2, FRB, FKBP or sgRNA Nm gBlocks)
- 09:29, 19 August 2016 (diff | hist) . . (+366) . . Team:Paris Saclay/Notebook/August/18 (→DreamTaq PCR on DH5a colonies transformed on the 17/08/2016 with PJet containing 1.2, FRB, FKBP or sgRNA Nm gBlocks)
- 09:15, 19 August 2016 (diff | hist) . . (+55) . . Team:Paris Saclay/Notebook/August/18 (→pPS16_002, pPS16_010, pPS16_013 and pPS16_014 PCR with pJET)
- 14:29, 16 August 2016 (diff | hist) . . (-1) . . Team:Paris Saclay/Notebook/August/12 (→PCR of pPS16_009)
- 14:29, 16 August 2016 (diff | hist) . . (+157) . . Team:Paris Saclay/Notebook/August/12 (→PCR of pPS16_009)
- 14:27, 16 August 2016 (diff | hist) . . (+78) . . N File:T--Paris Saclay--120826 visualization PCRGFP.jpg (Electrophoresis of PCR products, using primers IPS 83 and IPS 84 on pPS16_016.) (current)
- 08:46, 11 August 2016 (diff | hist) . . (+59) . . Team:Paris Saclay/Notebook/August/11 (→Transformation of DH5a cells with pPS16_009)
- 08:45, 11 August 2016 (diff | hist) . . (+331) . . N Team:Paris Saclay/Notebook/August/11 (Created page with "{{Team:Paris_Saclay/notebook_header}} = Wednesday 10<sup>th</sup> August= ==Lab work== ===Visualization=== ====Transformation of DH5a cells with pPS16_009==== ''By Léa'' Dh...")
- 08:37, 11 August 2016 (diff | hist) . . (+276) . . Team:Paris Saclay/Notebook/August/9 (→Tuesday 9th August)
- 14:56, 9 August 2016 (diff | hist) . . (+1) . . Team:Paris Saclay/Notebook/July/29 (→High fidelity PCR on bacteria transformed with pPS16_001, pPS16_002 and pPS16_005)
- 14:56, 9 August 2016 (diff | hist) . . (+32) . . N File:T--Paris Saclay--160729 Visualisation PCR3.jpeg (Migration of gBlocks PCR product) (current)
- 14:55, 9 August 2016 (diff | hist) . . (+1) . . Team:Paris Saclay/Notebook/July/29 (→High fidelity PCR on bacteria transformed with pPS16_001, pPS16_002 and pPS16_005)
- 14:53, 9 August 2016 (diff | hist) . . (-29) . . Team:Paris Saclay/Notebook/July/29 (→High fidelity PCR on bacteria transformed with pPS16_001, pPS16_002 and pPS16_005)
- 14:52, 9 August 2016 (diff | hist) . . (0) . . Team:Paris Saclay/Notebook/July/29 (→High fidelity PCR on bacteria transformed with pPS16_001, pPS16_002 and pPS16_005)
- 14:51, 9 August 2016 (diff | hist) . . (0) . . Team:Paris Saclay/Notebook/July/29 (→High fidelity PCR on bacteria transformed with pPS16_001, pPS16_002 and pPS16_005)
- 14:49, 9 August 2016 (diff | hist) . . (+28) . . Team:Paris Saclay/Notebook/July/29 (→High fidelity PCR on bacteria transformed with pPS16_001, pPS16_002 and pPS16_005)
- 14:49, 9 August 2016 (diff | hist) . . (+60) . . N File:T--Paris Saclay--160729 Visualisation PCR.tiff (Results of PCR product migration (gBlocks 1.1, 1.2, and 3.1)) (current)
- 14:46, 9 August 2016 (diff | hist) . . (0) . . Team:Paris Saclay/Notebook/July/29 (→High fidelity PCR on bacteria transformed with pPS16_001, pPS16_002 and pPS16_005)
- 14:43, 9 August 2016 (diff | hist) . . (+1) . . Team:Paris Saclay/Notebook/July/29 (→High fidelity PCR on bacteria transformed with pPS16_001, pPS16_002 and pPS16_005)
- 14:42, 9 August 2016 (diff | hist) . . (+104) . . Team:Paris Saclay/Notebook/July/29 (→High fidelity PCR on bacteria transformed with pPS16_001, pPS16_002 and pPS16_005)
- 14:22, 9 August 2016 (diff | hist) . . (+148) . . Team:Paris Saclay/Notebook/July/25 (→High fidelity Q5 PCR on bacteria transformed with pPS16_004, pPS16_007 and pPS16_008)
- 14:06, 9 August 2016 (diff | hist) . . (+132) . . Team:Paris Saclay/Notebook/July/26 (→Low Fidelity Dreamtaq PCR of DH5α|pPS16_002)
- 14:02, 9 August 2016 (diff | hist) . . (+1) . . Team:Paris Saclay/Notebook/July/26 (→High fidelity PCR on bacteria transformed with pPS16_001, pPS16_003, pPS16_005 and pPS16_009)
- 13:57, 9 August 2016 (diff | hist) . . (+158) . . Team:Paris Saclay/Notebook/July/26 (→High fidelity PCR on bacteria transformed with pPS16_001, pPS16_003, pPS16_005 and pPS16_009)
- 13:30, 9 August 2016 (diff | hist) . . (+55) . . N File:T--Paris Saclay--160726 visualization PCR2-2 4-1 4-2.jpeg (Results of PCR products (2.2, 4.1, 4.2) electrophoresis) (current)
- 13:28, 9 August 2016 (diff | hist) . . (+45) . . N File:T--Paris Saclay--160726 visualization PCR1-2.jpeg (Results of PCR products (1.2) electrophoresis) (current)
- 13:25, 9 August 2016 (diff | hist) . . (+68) . . N File:T--Paris Saclay--160726 visualization PCR1-1 2-1 3-1 GFP.jpeg (Results of PCR products (Gblocks 1.1, 2.1, 3.1, GFP) electrophoresis) (current)
- 14:33, 4 August 2016 (diff | hist) . . (+204) . . Team:Paris Saclay/Notebook/August/3 (→Tuesday 3st August)
- 12:57, 27 July 2016 (diff | hist) . . (+34) . . Team:Paris Saclay/Notebook/July/27 (→Transformation of DH5a cells)
- 12:56, 27 July 2016 (diff | hist) . . (+531) . . Team:Paris Saclay/Notebook/July/27 (→Wednesday 27th July)
- 08:24, 26 July 2016 (diff | hist) . . (+9) . . Team:Paris Saclay/Notebook/July/25 (→Characterization)
- 08:23, 26 July 2016 (diff | hist) . . (+173) . . Team:Paris Saclay/Notebook/July/25 (→Monday 25th July)
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