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White List (no Check-In required) |
Check-In Required (examples only!) |
Whole Organisms
(including viral genomes) |
- Risk Group 1 microorganisms
(For example: E. coli K-12, S. cerevisiae, B. subtilis, Lactobacillus spp.)
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- Risk Group 2 microorganisms
(For example: Pseudomonas spp.)
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- Bacteriophages T2, T4, T7, M13, P1, ΦX174 (Phi X 174), and λ (Lambda), unless containing a virulence factor (see below)
- Phagemids
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- Other viruses and bacteriophages
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- Human and primate cell lines that have been tested and certified free of known pathogens (consult your vendor; see FAQ)
- Cell lines from plants, fungi, or animals that are not primates (such as CHO cells or plant cells)
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- Human and primate cell lines that may contain pathogens
- All primary isolated cells (that is, cells taken directly from the body of a multicellular organism)
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- C. elegans (nematodes)
- Physcomitrella patens, Arabidopsis spp., Nicotiana spp.
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- Other multicellular organisms (animals, plants, insects, etc.)
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- ...and anything not explicitly listed
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| Parts |
All Registry parts, except those with a Red Flag placed by the Safety Committee |
Registry parts that have a Red Flag, which looks like this. A complete list of parts with Red Flags can be found here.
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Any part from a Risk Group 3 organism, regardless of its function |
Non-protein-coding parts in the following categories:
- Promoters, RBSes, Terminators
- Binding sites for transcriptional regulators, endonucleases, and other proteins that bind to DNA
- Aptamers and catalytic RNAs
- CRISPR guide RNAs, microRNAs, small interfering RNAs, and short hairpin RNAs that do not target human genes
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- CRISPR guide RNAs, microRNAs, small interfering RNAs, and short hairpin RNAs that target human genes
- Other non-protein-coding genes
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| Cas9, except when it is genomically integrated into a sexually reproducing organism |
Cas9 integrated into the genome of a sexually reproducing organism
(including organisms that reproduce both sexually and asexually, such as yeast) |
| Prions from non-mammalian organisms, such as yeast |
Prions from mammals, such as human PrP |
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Proteins or protein-coding genes from animals, plants, or Risk Group 1 / Risk Group 2 microorganisms, EXCEPT those in the list of "dangerous categories" on the right
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Proteins or protein-coding genes in the following dangerous categories:
- Virulence factors (see FAQ)
- Factors that help pathogens evade or shut down the immune system
- Factors that help pathogens halt the host's DNA/RNA replication, transcription, or translation
- Factors that regulate the immune system, such as cytokines and interferons
- Proteins that are toxic to humans
- Enzymes that produce a molecule that is toxic to humans
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...and anything not explicitly listed |
