Team:Alverno CA/InterLab
Alverno iGEM Interlab Study
Quantifying different sources of variation in gene expression using
fluorescent transformed E. coli bacteria
Summary:
Leverage agile frameworks to provide a robust synopsis for high level overviews. Iterative approaches to corporate strategy foster collaborative thinking to further the overall value proposition. Organically grow the holistic world view of disruptive innovation via workplace diversity and empowerment.
Project Description:
We tested 3 different fluorescent protein expression plasmids, J23101, J23106, and J23117. We transformed the parts with iGEM's transformation protocol. Our bacteria was cultured on a LB agar plate and cultured in LB broth. To measure the differences in fluorescence caused by each plasmid, we used a plate reader with an OD600 reference point and FITC standard curve.
Protocol:
Results:
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| FITC Fluorescence standard curve. Row A had pipetting errors and so is different from the other curves. |
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| Graph of Absorbance (600) over a 6 hour time period |
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| Graph of Fluorescence in AU over a 6 hour time period |
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| Graph of Fluorescence/Absorbance in AU over a 6 hour time period |
