Quantifying different sources of variation in gene expression using
fluorescent transformed E. coli bacteria
Summary:
Project Description:
We tested 3 different fluorescent protein expression plasmids, J23101, J23106, and J23117. We transformed the parts with iGEM's transformation protocol. Our bacteria was cultured on a LB agar plate and cultured in LB broth. To measure the differences in fluorescence caused by each plasmid, we used a plate reader with an OD600 reference point and FITC standard curve.
