Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Introduction Safety is a crucial issue in iGEM projects. As students imagine their own project, they have to make sure that their idea is not hazardous. Likewise, realization of the project in the lab is a second challenge for young students in terms of security. Working on living organisms and biological parts requires good laboratory practices in order to prevent spreading, contamination, or physical injuries. A good laboratory practice: To prevent disorder and accidents, the lab was divided in two parts : a wet laboratory for experiments and a dry laboratory for personal working. Students who were performing experiments were always wearing lab coats and kept experiment materials in the wet laboratory. Chemical product handling : Students who were experimenting sometimes had to use dangerous chemical products. Extra precautions were taken to handle them : {| class="wikitable" |- !Products !Dangers !Precautions !Usages |- |EtBr (Ethidium bromide) |mutagen agent cancer-causing agent |always manipulate EtBr wearing gloves add EtBr in cool solution only to prevent inhalation set up a special place and gather material to manipulate EtBr only |DNA visualization in agarose gels |- |Phenol/Chloroform |mutagen agent toxic agent irritant agent cancer-causing agent |always manipulate Phenol/Chloroform wearing gloves under a hood |DNA extraction |} On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can go beyond the questions on the safety forms, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.) Safe Project Design Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example: Choosing a non-pathogenic chassis Choosing parts that will not harm humans / animals / plants Substituting safer materials for dangerous materials in a proof-of-concept experiment Including an "induced lethality" or "kill-switch" device Safe Lab Work What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here! Safe Shipment Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?
Introduction
Safety is a crucial issue in iGEM projects. As students imagine their own project, they have to make sure that their idea is not hazardous. Likewise, realization of the project in the lab is a second challenge for young students in terms of security. Working on living organisms and biological parts requires good laboratory practices in order to prevent spreading, contamination, or physical injuries.
A good laboratory practice:
To prevent disorder and accidents, the lab was divided in two parts : a wet laboratory for experiments and a dry laboratory for personal working. Students who were performing experiments were always wearing lab coats and kept experiment materials in the wet laboratory.
Chemical product handling :
Students who were experimenting sometimes had to use dangerous chemical products. Extra precautions were taken to handle them : {| class="wikitable" |- !Products !Dangers !Precautions !Usages |- |EtBr (Ethidium bromide) |
mutagen agent
cancer-causing agent |
always manipulate EtBr wearing gloves
add EtBr in cool solution only to prevent inhalation
set up a special place and gather material to manipulate EtBr only |DNA visualization in agarose gels |- |Phenol/Chloroform |mutagen agent
toxic agent
irritant agent
cancer-causing agent |always manipulate Phenol/Chloroform wearing gloves under a hood |DNA extraction |}
On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can go beyond the questions on the safety forms, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)
Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:
What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!
Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?
