Team:Toronto/Testing

Notebook -include items in the LAB SECTION ONLY -include images -don’t include tables -don’t include morning/afternoon headings -point form, simplify if possible -use proper html language:

for months

for weeks

for dates
    unordered lists for all procedures
  • procedures are list items Remember to </close> all your items! <h3> June </h3> <h4> Week One </h4>
    07/06/16
    • Made 2 x 400mL 75% EtOH
    • Made 30 x 1mL Chloramphenicol (CAM) stocks
    • Created in silico primers for regular linear backbone
    08/06/16
    • Prepared and autoclaved LB broth
    • Prepared SOC media
    09/06/16
    • Autoclaved SOC media and aliquoted 30 x 1mL stocks
    • Obtained DH10β cells and made 5mL and 100mL overnight cultures
    10/06/16
    • Made TSS buffer
    • Grew cells to OD600 of 0.34
    • Made aliquots of chemically competent cells
    • Created small nickel plasmids
    <h4> Week Two
13/06/16
  • LB prep
  • Autoclaved 1.5mL microcentrifuge tubes
  • Carried out iGEM transformation efficiency protocol
  • Plated 17 plates with inoculating loops:
    • 3 x 0.5pg/uL
    • 3 x 5pg/uL
    • 3 x 10pg/uL