Team:Toronto/Testing

June

Week One

07/06/16

• Made 2 x 400mL 75% EtOH
• Made 30 x 1mL Chloramphenicol (CAM) stocks
• Created in silico primers for regular linear backbone

08/06/16

• Prepared and autoclaved LB broth
• Prepared SOC media

09/06/16

• Autoclaved SOC media and aliquoted 30 x 1mL stocks
• Obtained DH10β cells and made 5mL and 100mL overnight cultures

10/06/16

• Made TSS buffer
• Grew cells to OD600 of 0.34
• Made aliquots of chemically competent cells
• Created small nickel plasmids

Week Two

13/06/16

• LB prep
• Autoclaved 1.5mL microcentrifuge tubes
• Carried out iGEM transformation efficiency protocol
• Plated 17 plates with inoculating loops:
• 3 x 0.5pg/uL
• 3 x 5pg/uL
• 3 x 10pg/uL