Team:Bielefeld-CeBiTec/Notebook/Selection
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Irgentwas
Lab Notebook Selection
- Test
- Test
Transformation
| plasmid | organism / strain |
|---|---|
| pSB1C3 | E. coli DH5 α |
| pSB1C3 | E. coli DH5 α |
| pSB1C3 | E. coli DH5 α |
PCR
| polymerase | template | primer forward | primer reverse | Tm | time [min:s] |
|---|---|---|---|---|---|
Gibson
| DNA | concentration | length [bp] | volume [μL] |
|---|---|---|---|
restriction
| template | |
| enzyme 1 | |
| enzyme 2 |
Ligation
| DNA | concentration | length [bp] | volume [μL] |
|---|---|---|---|
