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Irgentwas
Lab Notebook Selection
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Transformation
plasmid | organism / strain |
---|---|
pSB1C3 | E. coli DH5 α |
pSB1C3 | E. coli DH5 α |
pSB1C3 | E. coli DH5 α |
PCR
polymerase | template | primer forward | primer reverse | Tm | time [min:s] |
---|---|---|---|---|---|
Gibson
DNA | concentration | length [bp] | volume [μL] |
---|---|---|---|
restriction
template | |
enzyme 1 | |
enzyme 2 |
Ligation
DNA | concentration | length [bp] | volume [μL] |
---|---|---|---|