Team:Danci-K8/Notebook
Danci-K8
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15/7/2016
In the lab today: Itay, May, Tomer
Planning synthetic DNA
Planning 8 synthetic SNPs and placing an order.
16/7/2016
In the lab today: Itay, May, Tomer
Planning synthetic DNA
Planning 7 synthetic SNPs and placing an order.
The final SNPs (Single nucleotide polymorphism)
Final Order Table
| Number | Taste | RS |
|---|---|---|
| 1 | Sweet | rs35874116 |
| 2 | Sweet | rs307355 |
| 3 | PTC | rs713598 |
| 4 | PTC | rs1726866 |
| 5 | PTC | rs1024639 |
| 6 | Consuming Alcohol | rs846672 |
| 7 | Coffee Bitterness | RS765007 |
| 8 | Coffee Bitterness | RS2234001 |
| 9 | Coffee Bitterness | RS2234012 |
| 10 | Coffee Bitterness | RS2227264 |
| 11 | Grapefruit juice bitterness | rs10772420 |
| 12 | Cilantro Preference | rs72921001 |
| 13 | Taste of NaC1 & Sour | rs17492553 |
| 14 | Taste of NaC1 & Sour | rs34160967 |
| 16 | Umami& Monosodium gluten | rs76755863 |
19/7/2016
In the lab today: Avital, Gal, Lidor
Planning 5 gRNA for CRISPR reaction
| Taste/NoTaste | Spacer | PAM |
|---|---|---|
| No Taste | TCAGTTTTGTGGCAGATGAC | AGG |
| No Taste | CACATCAGTGAGGAGATTCT | AGG |
| No Taste | TCGTGTGTGTCATGAAAGCT | TGG |
| No Taste | GGCCACGGGCACGGTTGTAGC | CGG |
| (non targeting) | aggatcacct gagtccaggg | - |
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