Team:UGent Belgium/LabNotebook

Bootstrap 101 Template



MENU ▤

Lab Notebook

August

  • August 29:

    • We made a strong expression vector (pXS) by restriction of the linearized plasmid backbone pSB1C3 and insertion of gblock made up of promoter-RBS-cloning site-terminator-terminator (cut with EcoRI and PstI)
    • Our first attempts to isolate the inaZ gene from P. syringae and the inaX gene from X. campestrisfailed
  • August 30:

    • We transformed the strong expression vector (cf. 29/08) by electroshock into E. coli TOP10
    • As a backup strategy we also used CPEC with the backbone from the K584027 plasmid to generate a strong expression vector.
  • August 31:

    • Second attempt to isolate the inaZ gene from P. syringae and the inaX gene from X. campestris. InaX failed, but inaZ was successful
    • Transformation of the strong expression vector by restriction and ligation was not very successful, we only had a few colonies. The backbone amplification using CPEC was successful (cf 30/08).