Team:Aachen/InterLab
InterLab Study 2016
The 3rd International InterLab Study of the iGEM competition sought to compare the results of certain measurements conducted by on different devices by different scientists in different labs all over the world. In order to have a distinctive set of measurable parameters, the expression of GFP (I13504) under the control of three promotors with different strengths was subject to measurement. The promotors are part of a constitutive promotor family and where constructed by John Anderson in 2006 and added to the iGEM Registry. In this year’s InterLab study the following members of this promotor family where investigated:J23101 (Device 1, strong), J23106 (Device 2, medium) and J23117 (Device 3, weak). As a negative control the TetR repressible promotor (R0040) was used. However, with the positive control we had some issues. Originally the positive control should have been GFP under the control of the J23151 promotor (I20270) but apparently this construct was neither in the InterLab measurement kit (tube was empty) nor in the respective backup well in the distribution (Kit Plate 3, well 8P). We didn´t have the time left to build the positive control from scratch or identify which construct it actually was, but after communication with iGEM HQ we used it anyway.
