Team:Cardiff Wales/Collaborations

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Collaborations

During our project we have had discussions with numerous teams, usually over Skpe or on twitter!

These discussions have involved teams based in:

> Washington University in St Louis, USA
> Paris-Sacley, France
> Oxford University, UK
> Trinity College in Dublin, Ireland

FLIM experiments with Oxford iGEM

The Oxford iGEM team wanted to test their biobricks using Fluorescence Lifetime IMaging (FLIM). The Cardiff Bioimaging facility has a microscope that can perform these reactions so we offered to test their samples.

They provided bacteria containing three constructs that we grew overnight in the presence of 5uM copper sulphate -/+ 2mM Arabinose (which induced expression using the pBAD promotor).

These constructs were:

1. A constitutive GFP

2. Inducible CG:GFP cooper chelator protein (BioBrick Link

3. Inducible MG:GFP cooper chelator protein

With help of Dr Anthony Hayes in the Cardiff Biomaging facility we obtained the following FLIM data for their constructs. This has helped with their characterisation as described on their Wiki

GFP-Cu chelator FLIM experiment

Tables shows triplicate (i.e. 3 regions per cover-slipped area) values of the peak lifetimes resulting from measurements using Excitation=483nm, Emission=500-550nm.

Construct	Arabinose -ve	Arabinose +ve
Constitutive GFP Expression	2.6ns, 2.6ns, 2.6ns	2.6ns, 2.6ns, 2.6ns
pBAD-CG::GFP	3.3ns, 3.3ns, 3.3ns	2.5ns, 2.5ns, 2.5ns
pBAD-MG::GFP	2.6ns, 2.8ns, 2.6ns	2.2ns, 2.3ns, 2.3ns

Include an image if available!

Survey Participation

★ ALERT!

This page is used by the judges to evaluate your team for the team collaboration silver medal criterion.

Delete this box in order to be evaluated for this medal. See more information at Instructions for Evaluated Pages .

Sharing and collaboration are core values of iGEM. We encourage you to reach out and work with other teams on difficult problems that you can more easily solve together.

Which other teams can we work with?

You can work with any other team in the competition, including software, hardware, high school and other tracks. You can also work with non-iGEM research groups, but they do not count towards the iGEM team collaboration silver medal criterion.

In order to meet the silver medal criteria on helping another team, you must complete this page and detail the nature of your collaboration with another iGEM team.

Here are some suggestions for projects you could work on with other teams:

• Improve the function of another team's BioBrick Part or Device
• Characterize another team's part
• Debug a construct
• Model or simulating another team's system
• Test another team's software
• Help build and test another team's hardware project
• Mentor a high-school team