Team:OUC-China/Protocol

1. Elementary Operation

1) Luria-Bertani liquid and Luria-Bertani solid medium
2) Inoculation
3) Polymerase Chain Reaction
4) Gel Electrophoresis
5) Restriction Enzyme Digestion
6) Gel Extraction
7) Ligation system
8) Transformation of E.coli
9) Conservation
10) Sterilization
11) Plasmid extraction

2. Plasmid Construction

1) Overlap PCR
2) Gibson
3) DNA cassettes

3. Systems detection

1) RNA extraction
2) Reserve transcription
3) Quantitative Real-time PCR
4) Fluorescence measurement protocol

Cistrons Concerto

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