Team:UofC Calgary/Attributions
Attributions
|
(Dr./Mr./Mrs.) Name/Affiliation: |
Contribution to the team: |
|
Dr. Susan Lees-Miller Professor, Departments of Biochemistry and Molecular Biology |
Dr. Lees-Miller’s lab kindly donated to us the mammalian cell line (HCT116) and gave us access to her gamma cell irradiator, both of which were a great help to us in characterizing BBI. |
|
Dr. Aaron Goodarzi Assistant Professor, Departments of Biochemistry & Molecular Biology |
Dr. Aaron Goodarzi was a great help in conceptualizing the project, from introducing us to soybean derived radioprotective peptides to helping us plan the experiments we conducted to characterize BBI. Dr.Goodarzi also kindly donated the primary fibroblast cell line (1BR3) and trained us in conducting an H2AX foci assay. He was critical to the characterization of BBI. |
|
Dr. Sui-Lam Wong |
Dr. Wong provided us with protocols for growth media, super rich media, sporulation media, 2X SG media , competency and glycerol stock protocols for B. subtilis. He kindly gave us B. subtilis WB800 strain to with for our project.
|
|
Dr. Craig Jenne and Members of the Jenne Lab |
Dr. Jenne has a license to work with mice for scientific purpose. He has assisted the team by submitting the ethics approval for in vivo testing of the patch, BBI, and B. subtilis. Members of the UCalgary iGEM team have filled out the ethics application but it was submitted under Dr. Jenne’s name. Once, ethics approval was received, Dr. Jenne kindle donated 18 BALB/c mice for the experiment. Rachelle Davis (Graduate Student) and Mandy Tse (Lab Manager) did all the mice handling work(put the patches on, regular check up on mice health and behavior, blood extraction, and euthanization). Dr. Seok-Joo Kim (Post- Doctoral Fellow) and Dr. Jenne assisted in mice tissue analyses (staining …..) |
|
Daniel Ziemianowicz Ph.D., Graduate Student Advisor |
Daniel was a dedicated supporter of the group who had previous experience of competing in iGEM, who was familiar in many of the different parts of a project and provided coaching to the various subgroups working on different objectives. He also used his experience in mass spectroscopy to help us with two important parts of our project. |
|
Dennis Kim iGEM 2014 Team BHSc. Undergraduate Student Advisor
|
Dennis assisted the team in troubleshooting for ComK transfromations and integration into Bacillus subtilis. |
|
Nic Sieben _______________
|
He provided the team with plasmid miniprep protocol. |
|
Rai Chaudhuri iGEM 2014 Team MSc. Graduate Student Advisor |
Rai assisted the team with chassis experiment troubleshooting. |
|
Agnes Klein Director of Centre of Evaluation of Radiopharmaceuticals and Biotherapeutics Health Canada |
Agnes Klein, Dr. Ting, Dr. Glennon, Dr. Hagen looked over our policy brief on regulations on cell- based therapeutics in Canada. |
|
Dr. Walter Glennon Bioethicist/Neuroethicist at the University of Calgary |
|
|
Dr Gregory Hagen Associate Professor, Associate Dean (Research) at University of Calgary |
|
|
Dr. Fabiola Ting Instructor, Community of Health Sciences University of Calgary |
|
|
Eduardo and Nicholas __________________ |
They planned and executed a Tom Baker Cancer Institute Tour for our team. |
|
Minds in Motion Calgary |
They provided the team with an outreach opportunity to interact and educate children between the ages of 10-14 years. |
|
Beakerhead Calgary |
We were provided with an outreach opportunity to interact with children and adults with Bacterial painting. |
|
Telus Spark Calgary |
Telus Spark provided the team with an outreach opportunity to educate adults about synthetic biology through their Hack It Adults Night. |
|
Guanajuato Mexico 2016 iGEM Team |
Collaborated with the Mexico iGEM team to create and execute a curriculum to educate first year university students on the basis of synthetic biology. |
|
University of Rice 2016 iGEM Team
|
Contributed to their Humans of Synthetic Biology project by providing them with the story of why one of our team members joined iGEM. |
|
Mind Fuel Calgary |
Provided the team with the opportunity to meet with international iGEM judges and receive critical feedback on our project. Also, they worked with us to plan and execute aGEM, a competition between all Alberta iGEM high school and collegiate teams. |
|
Dr. Amir Nezhad |
Dr. Nezhad offered counselling on the early development of the project and referred various professor to us for resources |
|
Dr. Colin Dalton |
Dr. Dalton gave us an extensive insight into microneedles and their inadequacy for the objective of the project and advised the change of design to a transdermal patch |
|
Dr. Uttandaraman (U.T.) Sundararaj |
Dr. Sundararaj offered technical support with the prototype by providing us insights on thermoforming and properties of polymers and how we can incorporate these methods for small scale production. |
|
3M |
3M provided the materials for making the prototype for mouse testing and fabricated a prototype in their lab for the team |
|
Dow Corning |
Dow Corning provided adhesives for making the prototype for mouse testing |
|
Robert Mayall |
Offered counsel on the membrane experiment and alternative materials |
|
Patrick Wu |
iGEM design seminar on Wiki design, and visual presentation tips. |
|
Dr. Justin MacCallum |
Modelling simulations. |
|
Nicholas Jette Ph.D., Graduate Student Advisor |
Nicholas was a great aid in the execution of the assays that we wished to run. From giving us training in tissue culture, to teaching how to do the clonogenics assay, Nicholas was a great help in characterizing BBI. |
|
Shaun Moore Ph.D., Graduate Student |
Shaun provided us with advice in conducting the double strand break assay and helped us with the troubleshooting of our assays. |
|
Karolin Klement Ph.D. Post Doctoral Fellow |
Karolin helped us with the clonogenics assay and provided us with advice when we changed our cell lines from HCT116 to 1BR3. Since primary cell lines have different requirements than cancer cells, she showed us what was required to run a clonogenic assay with 1BR3. |
|
Alina Kunitskaya Advisor
|
Alina had mentored the device subgroup by providing guidance on the design process, troubleshooting visual modelling and providing alternative opinions. She also acted as our contact within NASA and helped to schedule a meeting with Dr. Cagle |
Team
|
Dr. Mayi Arcellana-Panlilio Adjunct Associate Professor, Biochemistry & Molecular Biology |
|
|
Nicholas D’Aleo-Sotas BSc. Cellular Molecular Microbial Biology, Team Member |
Worked on HCT116 and Fibroblast clonogenics. Tissue culture guru. ComK transformation into Bacillus subtilis savant. |
|
Nilesh Sharma BSc. Neuroscience Team Member |
Worked in immunoflourescence assay with cell fixing and antibody staining. Worked in prototype testing, writing ethics application and observing patch application. Also worked on mathematical modelling of how BBI diffuses through the skin and on molecular simulations of BBI movement through a lipid bilayer. |
|
Miriam Li BHSc. Biomedical Sciences Team Member |
Worked on HCT116 and 1BR3 clonogenics assays. Oversaw mice trials including administration of patch and blood draws. Also worked in Human Practices in writing up the whole policy brief, setting up meetings with professionals, and doing write-ups. |
|
Syed Jafri BHSc. Bioinformatics Team Member |
Coded the Wiki. Worked in immunofluorescence assays using γ-H2AX and 53BP1 antibodies to visualize DSBs. |
|
Sid Goutam BHSc. Biomedical Sciences Team Member |
Worked on the clonogenic assay with HCT116 cells. Worked on the double strand break assay using immunofluorescence. Took part in mathematical modeling. |
|
Nishi Patel BSc. Cellular Molecular Microbial Biology Team Member |
Designed genetic constructs for BBI and the indicator system. Worked with the Jenne lab for ethics approval and in vivo testing of transdermal patches in mice. Oversaw the manufacture, administration of patches. Analyzed mice tissue for any immunological responses. For Human Practices, consulted on the policy brief and helped set up meetings. |
|
James Johnston BHSc. Biomedical Sciences Team Member |
Worked on determining the growth of Bacillus with Rachelle. Worked on the cloning and eventual expression of BBI into both E. coli and Bacillus subtilis. I also helped with the layout and coding of the wiki. |
|
Rachelle Varga BSc. Cellular, Molecular, and Microbial Biology Team Member |
Determined growth rates of B. subtilis at varying temperatures and using different media. Worked on developing usable ComK genetic construct and cloning it in E. coli and B. subtilis. Organized outreach events. Ran all forms of social media (Facebook, Twitter, and Instagram). |
|
Elena Fekete BSc. Cellular, Molecular, and Microbial Biology Team Member |
Planned outreach activities, and worked on research and editing for the policy brief. Helped to set up interviews with professionals. |
|
Shalpinder Dhothar BSc. Biological Sciences Team Member |
Arranged and conducted meetings with professionals, planned outreach programs, assisted with collaboration work, and worked on the research, writing and editing of the policy brief. |
|
Christine Phan BSc. Biological Sciences Team Member |
Performed assays for material testing, created Solidworks model of the design, created Autodesk Maya animation of the system and collaborated on the design process for the physical delivery system. Designed wiki wireframes as well as slide deck for the presentation. |
|
Tiffany Dang BSc. Chemical and Biomedical Engineering Team Member |
Collaborated on the design process for the physical delivery system, created Solidworks model of the design, and performed material testing. For Human Practices, set up meeting with professionals, consulted on the policy brief, helped with outreach programs and organized collaboration. |
|
David Nguyen BSc. Chemical and Petroleum Engineering Team Member |
Undertook material research, reached out to companies to obtain materials, designed and fabricated a physical prototype for the mouse testing, initialized the diffusion mathematical model and provided support throughout the project, compiled device subteam notebook and results as well as applying for travel cost funding |
|
Neliza Mendoza BSc. Civil Engineering Team Member |
Took charge of the analytical modelling for diffusion of drug through skin using MATLAB, performed adhesive assays for patch laminates used in prototyping, and compiled an information manual for our biotherapeutic device for potential user reference. Certified meeting organizer. |
|
Noshin Karim BSc. Mechanical Engineering Team Member |
Took charge of the numerical modelling of diffusion of peptide through the physical delivery system using MATLAB. Led discussions on design process for the physical delivery system, researched into materials, helped with developing SolidWorks model of design. Combined mathematical model and lab experiment results to determine how the patch works. |
