Team:CLSB-UK/Parts
In the Lab
At the end of the day, an iGEM team’s project is made or broken in the lab. And at CLSB, if you were to walk along the science corridor to the small, unassuming lab that is Mr Zivanic’s, in the months leading up to Jamboree, be it before school or after, during term time or while the students are meant to be off school, you would undoubtedly find it bustling with activity. For this is where the iGEM team made our home over the last year. This is where we developed from a team that marvelled at the accuracy of our micropipettes and struggled to put on microbiology lab coats to one that routinely performed gel extractions with ease, and confidently recorded the growth rate of our cyanobacteria. We came from humble beginnings, but by soldiering on past cells that demanded -80ºC freezers and ligations that refused to yield any results for three weeks in a row, by coming in at the crack of dawn and leaving after the sun had long since set, by sacrificing our well earned summer rest while our friends went off on holiday, we have achieved more than we could ever have hoped for.
Parts created by CLSB-UK team
We have created the following parts for our project:
BBa_K2078000
As the suitable bicarbonate transporter gene didn't exist in the registry before, we decided that it would be useful to add this part as it would enable other groups working with this chassis to use it.
Figure 1. Cloning strategy used to produce BBa_K2078000 part. cmpA was ordered from IDT as a G-block together with the forward and reverse primers. It was amplifies using PCR thermocycler.
BBa_K2078001
Parts available for Synechocystis PCC6803 chassis are few and far between, so we struggled to find available promoter that was suitable for our project. This is why we opted for an E.coli promoter in the hope that it would work in Synechocystis, too.
Figure 2. Cloning strategy used to produce BBa_K2078001 part.
BBa_K2078002
Details of how this part was made
Figure 3. Cloning strategy used to produce BBa_K2078002 part.
