★ Basic parts designed by iGEM Aix Marseille 2016
You will find on this page the basic parts which have been designed by our team. For further details, you can also go on http://www.parts.igem.org and have a look to the biobrick reference BBa_ basic part special prize.
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A basic part is a functional unit of DNA that cannot be subdivided into smaller component parts. BBa_R0051 is an example of a basic part, a promoter regulated by lambda cl.
Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are many opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts.
des A, lysine decarboxylase coding sequence (BbaK1951000)
This DNA sequence codes Lysine descarboxylase (Streptomyces coelicolor) is an enzyme from the lyase family that converts lysine to cadaverin. The enzyme realizes the carbonyl group of the lysin amino acid. There is cadeverin producing (1,5-diaminopentane), a primary diamine which alkaline environment. The lysine decarboxylase is an enzyme induced the synthesis of which is promoted by anaerobiosis and an acidic pH.
In bacteriology, this enzyme is sought through the middle of Moeller lysine or medium lysine Taylor. This enzyme is also the first step in the production of desferrioxame B which is a siderophore.
des B, monooxygenase coding sequence (BbaK1951001)
This sequence codes Monooxygenase which is an enzyme that incorporate one hydroxyl group into substrates in many metabolic pathways. In this reaction, the two atoms of dioxygen are reduced to one hydroxyl and one H2O molecule by the concomitant oxidation of NAD(P)H. It is also the second step in the production of desferrioxamine B in Streptomyces coelicolor, allowing the transformation of cadeverine into N-hydroxycadaverine
des C, acyl transferase coding sequence (BbaK1951002)
desC is the coding sequence of DesC which is an acyl transferase of the Desferrioxamine production pathway. It is the enzyme of the third step that transform N-hydroxycadaverin into N-acetyl N-hydroxucadaverine by an Acetyl-CoA dependent manner.
des D, desferrioxamine biosynthesis coding sequence (BbaK1951003)
This coding sequence codes DesD of Streptomyces coelicolor, which is the last protein involved in the metabolic pathway of Desferrioxamine B. This enzyme is called desferrioxamine biosynthesis protein or DesD and allows to transform N-acetyl N-hydroxycadaverine into Desferrioxamine B (by the transformation of 3a nucleoside triphosphate into 3a nucleoside 5'monophosphate-3-diphosphate).