Composite part
Composite parts we have designed
BBa_K1951004 : DesA producer
Part Composition:
This part is composed of 3 subparts :
- RBS: Ribosome Binding Site
- pARA/araC inductible promoter
- Lysine decarboxylase
Lysine decarboxylase DesA:
Lysine descarboxylase from Streptomyces coelicolor is an enzyme from the lyase family that converts lysine to cadaverin. The enzyme realizes the carbonyl group of the lysin amino acid. Cadaverine(1,5-diaminopentane) is a primary diamine which alkaline environment. The lysine decarboxylase is an enzyme induced the synthesis of which is promoted by anaerobiosis and an acidic pH. In bacteriology, this enzyme is sought through the middle of Moeller lysine or medium lysine Taylor. We registered the original sequence of this subpart in the iGEM registry of standard parts (BBa_K1951000). We optimized our sequence for E.coli and ordered the synthesis by addition of an inductible promoter.
