Thursday, June 23rd
Results:
Kelly’s competent cells performed well; ours did not. The iGEM DNA was very dilute. We have no data from the negative control, and we’re using results from Kelly’s cells.
Figure 1: Transformation plates
Figure 1(cont): Transformation plates
Tasks:
Sara
- Background research on alternate membrane proteins
- Looked for parts
Tasfia
- Researched on history of getting ClyA into membranes in vivo
- Learned how to use Genome compiler
Michelle
- CSS research for the website
- Background research
- Worked on constructs
Tyler
- Researched ClyA’s orientation and how to reduce toxicity
- Presentation for tomorrow
- ClyA-His6 part
Sam
- Designed the Tat-his6 part
Jordan
- Picked colonies from the transformation from the night before
- Innoculated more cells
