Friday, July 1st
Agenda:
- Constructs
- Practice presentations
- Ligation
- Meeting with grad students
Results:
Cloning practice: Redid the gel from yesterday. This time, we didn't run the gel quite long enough and the bands didn't separate that much.
Tasks:
Michelle
- Website research
- Golden Gate preparation
Sara
- Ran the gel again
- Emailed Bernd
- Looked into choosing homologous regions to add onto the signaling sequences, ran into problems with annealing temp
Tasfia
- Attempted to make overhangs on SS parts, but the Tm was too high for optimal Gibson assembly with really long overhangs
- Cloning practice
Tyler
- Finalized homology arms for split Cas9
- Designed RTW PCR primers of tet backbone
- Received Promega’s order of restriction
Jordan
- Helped with gels
- Crowdfunding got approved
- Edited crowdfunding page
- Presented to grad students
Paul
- Update presentation with GFP fusions
- Researched GFP fusions
- Upload sfGFP to Genome Compiler
