Team:Paris Bettencourt/Project/Indigo

Project
- Overview
- Assay
- Microbiology
- Enzyme
- Binding
- Indigo
- Model
Practices
- About Perc
- Dry Cleaners
- Design
- Events
Safety
- Lab Safety
- Product
  Safety
Achievements
Notebook
Team

@iGEM_Paris

Making patterns on denim

Goals

Find microorganisms that naturally degrade indigo
Test enzyme known for degrading indigo
Make patterns on jeans

Results

3 strains degrading indigo in liquid M9 were identified

We exported the idea of cleaning stains with biology and our methods to do so into a more artistic approach with the indigo part of our project. Our goal is to find microorganisms and enzymes that can degrade indigo and to test them on denim. The final aim will be to use them to make patterns on jeans.

We basically reproduce the work of enzyme and microbe’s team in a smaller scale: through literature we identified one interesting enzyme that we tested, and we collected different microbes with potential capacity of degradation of indigo.

Highlights

Goals

Methods

Results

BioBrick

Abstract

In this subproject Mission Indigo, we wanted to export our idea of degrading stains with biology and our methods in a more artistic and fashionable approach: degrading indigo on jeans to make patterns (just like jeans tye and dye!). We worked just as the team did with anthocyanin to find ways to degrade indigo: investigate already know enzymes that degrade our pigment and find microorganisms that naturally degrade it. We basically reproduce the work of enzyme and microbe’s team in a smaller scale: through literature we identified one interesting enzyme that we tested, and we collected different microbes with potential capacity of degradation of indigo.
We cultured microorganisms on denim covered with minimal M9, isolated the strains and identified them. Then, we tested their capacity at indigo’s degradation. At the end we have three microorganisms that degrade indigo: 2 Streptomyces and Pantoea.