Team:XJTLU-CHINA/Attributions
Attribution
· XJTLU-CHINA
Members in XJTLU-CHINA are
responsible for the project in 2016 and handled almost all issues. However,
there are also great supports from the surroundings. We would like to say many
thanks to instructors, sponsors, practical consultants (including students), the
public in human practice, etc.
Member’s Attribution
Overview
We started our brainstorming about
this year project in December, 2015 and started the wet experiments in May,
2016. The lab experiment lasted for about 7 months.
Distribution
Management:
Wenbo Xu
Wenbo Xu almost managed all the
events in XJTLU-CHINA and participated in both dry and wet experiments.
Overall
idea: Wenbo Xu, Ziheng Chen
The idea of Qβ replicase was
firstly put forward by Wenbo Xu and the idea of intron by Ziheng Chen. Other
members developed their ideas and had brainstorming together to design the
specific outline of this project in the later processing.
Wet
Experiments
Almost all members in XJTLU-CHINA
were involved in wet experiments. The main members in each group are listed
below and they had also been involved in dry experiment to some
degree for the specific design of each part.
1.
Ts
Tu Rep construction: Mulei Gong, Xiaoyu
Li, Yuntao Wang, Zhiming Mao Kunqi Chen
2.
Intron
construction: Yuming Xu, Hongyu Duan,
Kaitong Lu, Fengshuo Liu, Ziheng Chen, Zhiming Mao
3.
Protein
detection and characterization: Qing
Zhang, Mengjia Li
4.
Qβ
Genome deletion and reconstitution: Yizhe
Mao, Chengyang Nie, Fengshuo Liu
5.
Retrohoming:
Fengshuo Liu, Hongyu Duan, Kaitong Lu, Yizhe
Mao
6.
Improvement
of last year project: Hongyu Duan, Kaitong
Lu, Chengyang Nie
Mathematical
modeling
Kaifu
Wang
established all mathematical models for the whole project and he helped process
the data of DNA sequences and understand the mathematical part in references.
Art
and Accessory
Poster: Yuming Xu, Yuntao Wang
Propaganda Video: Zhiming Mao,
Ziheng Chen
Uniform: Yuming Xu
Wiki
Webpage: Dixing Xu
Element design (figures): Yuming
Xu, Yuntao Wang
Content writing: Xiaoyu Li, Ziheng
Chen, Mengjia Li, Mulei Gong, Zhiming Mao
Human
Practice
Undergraduate
student research and novel Biology
All members participated in public
speeches.
Presenter: Zhiming Mao Mulei Gong,
Wenbo Xu
Invited Professor: Dr. Jia
Meng & Dr. Tatsuhiko Kadowaki
2016
International STEM Festival
Almost all members participated in
STEM Festival as narrators to introduce our 2016 project and the previous
project to the public.
Public speech presenters: Yuntao
Wang, Mulei Gong, Hongyu Duan
Road
show in Shanghai Technology Museum:
Zhiming Mao, Kaitong Lu, Fengshuo Liu,
Ziheng Chen
Collaboration
Liaison With other iGEM teams
seeking for collaboration: Yuntao Wang, Qing Zhang, Zhiming Mao
·With ZJU iGEM
team: Qing Zhang
Qing Zhang helped ZJU to improve
their hardware and characterize a part. She worked with infoCo club of XJTLU to
construct a programmable red-green lighting system suitable for 96-well plate,
which enables them to remotely control the on-off setting and light intensity
for each sample space. This extendable design may accelerate their experiment
and it shows a connection between how arduino (electric engineering) can help
to characterize bioengineering process.
·Interlab: Qing Zhang, Mengjia Li
Qing Zhang and Mengjia Li measured
the relative fluorescence of GFP of 5 devices, which is aimed to characterize
the measurement of fluorescence.
·STEM Festival: Wenbo Xu
In STEM Festival, XJTLU-CHINA was
collaborated with ZJU and NYU-Shanghai and did a group presentation towards the
public.
· Demonstration in
Shanghai Technology Museum: Zhiming Mao
XJTLU-CHINA was collaborated with
SJTU-Shanghai, Tongji University, NYU-Shanghai for a road show.
Additional Supports
Instructor:
Dechang
Xu, Guoxia Han
Fundraising
help and advice:
Department of Biological Sciences, XJTLU
Lab
support: Teaching lab in XJTLU
Lab
supervisor: Zhongkai Huang
Thanks
for the following outside support to XJTLU-CHINA:
Snapgene
provided us with free online access.
Material
Support, technical support and order support: Symbio-tech
Company
HB
101 cells: from Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese
Academy of Sciences
Plasmids
for Qbeta 7 plasmid: Associate professor Charlotte Rohde
Knudsen, Department of Molecular Biology and Genetics, Aarhus University
