Design
Our lab experience enabled us to create the final Biobrocks of our project which are a siderophore Desferrioxamine B producer and a flagellin producer.
MObilisation result
===Proof of protein production===
Production of every enzymes involved in the pathway has been shown by a SDS page and Comassie Blue. We compared the production of proteins in different background :
- E. coli Tg1 wuthout any plasmide - E. coli Tg1 without pSB1C3 containing the RFP coding sequence - E. coli complemented with our biobrick Bba_K1951011 before and after induction. (you can observe the production of the 4 proteins on the figure below on the left; right : before induction, left : after induction)
Proof of fonctionnality
We investigated if DesA (Lysine decarboxylase) was fonctionnal, by measurement of cadaverine using HPLC with C18 column and proofed our biobrick is a producer of this protein and makes it fonctionnal.
In the futur, we plan to test the whole pathway viability in the first hand. In a second hand, the potential adsorption of platium by our siderophore would be investigate in a rich metal medium.
Biosorption result
Here you can describe the results of your project and your future plans.
What should this page contain?
- Clearly and objectively describe the results of your work.
- Future plans for the project
- Considerations for replicating the experiments
Project Achievements
You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.
- A list of linked bullet points of the successful results during your project
- A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.
Inspiration
See how other teams presented their results.
- <a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a>
- <a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a>
- <a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a>
