Welcome to iGEM Aachen 2016
| BioBrick |
Description |
| K2020000 |
subtilisin E gene, optimized for E. coli codon usage |
| K2020001 |
subtilisin E gene, optimized for E. coli codon usage, with leader sequence pelB |
| K2020002 |
expression system of subtilisin E in E. coli |
| K2020003 |
mutated version of expression system of subtilisin E in E. coli (S221Y) |
| K2020004 |
mutated version of expression system of subtilisin E in E. coli (S221stop) |
| K2020005 |
mutated version of expression system of subtilisin E in E. coli (Y-1W) |
| K2020006 |
mutated version of expression system of subtilisin E in E. coli (Y-1stop) |
| K2020020 |
subtilisin E gene, optimized for S. cerevisiae codon usage |
| K2020026 |
leader sequence MFalpha (different version of the biobrick (K792002) |
| K2020027 |
leader sequence MFalpha (BBa_K2020026) plus fluorescence protein RFP (BBa_E2060) |
| K2020028 |
leader sequence MFalpha (BBa_K2020026) plus fluorescence protein YFP (BBa_K165005) |
| K2020029 |
leader sequence MFalpha (BBa_K2020026) plus fluorescence protein mOrange (BBa_E2050) |
| K2020040 |
screening plasmid for incorporation of non-canonical amino acids → pRXG (twin pFRY) |
| K2020041 |
screening plasmid for incorporation of non-canonical amino acids → pRYG (twin pFRYC) |
| K2020042 |
tRNA specific for tyrosine and UAG codon in E. coli |
| K2020043 |
tRNA synthetase specific for the ncAA AzF and UAG codon in E. coli → AzF-RS |
| K2020047 |
tRNA synthetase specific for the ncAA 5HTP and UAG codon in E. coli → 5HTP-RS |
| K2020050 |
tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS |
| K2020051 |
mutated tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS with Y32G |
| K2020052 |
tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 1 |
| K2020053 |
tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 2 |
| K2020054 |
tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 3 |
| K2020055 |
tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 4 |
| K2020056 |
tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 5 |
| K2020057 |
tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 6 |
| K2020058 |
tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 7 |
| K2020059 |
tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 8 |
| K2020060 |
tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 9 |
| K2020061 |
tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 10 |
Basic Building Blocks
Mutated Versions
Basic Building Blocks
Mutated Versions
Improvement of an Existing Part
Screening System
tRNA and Synthetases
