Team:Aachen/Basic Part
Parts
| BioBrick | Description |
| K2020000 | subtilisin E gene, optimized for E. coli codon usage |
| K2020001 | subtilisin E gene, optimized for E. coli codon usage, with leader sequence pelB |
| K2020002 | expression system for subtilisin E in E. coli |
| K2020003 | mutated expression system for subtilisin E in E. coli (S221Y) |
| K2020004 | mutated expression system for subtilisin E in E. coli (S221X) |
| K2020005 | mutated expression system for subtilisin E in E. coli (Y77W) |
| K2020006 | mutated expression system for subtilisin E in E. coli (Y77X) |
| K2020020 | subtilisin E gene, optimized for S. cerevisiae codon usage |
| K2020026 | leader sequence MFalpha (different version of the biobrick (K792002) |
| K2020027 | leader sequence MFalpha (BBa_K2020026) plus fluorescence protein RFP (BBa_E2060) |
| K2020028 | leader sequence MFalpha (BBa_K2020026) plus fluorescence protein YFP (BBa_K165005) |
| K2020029 | leader sequence MFalpha (BBa_K2020026) plus fluorescence protein mOrange (BBa_E2050) |
| K2020040 | screening plasmid for incorporation of non-canonical amino acids → pRXG (twin pFRY) |
| K2020041 | screening plasmid for incorporation of non-canonical amino acids → pRYG (twin pFRYC) |
| K2020042 | tRNA specific for tyrosine and UAG codon in E. coli |
| K2020043 | tRNA synthetase specific for the ncAA AzF and UAG codon in E. coli → AzF-RS |
| K2020047 | tRNA synthetase specific for the ncAA 5HTP and UAG codon in E. coli → 5HTP-RS |
| K2020050 | tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS |
| K2020051 | mutated tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS with Y32G |
| K2020052 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 1 |
| K2020053 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 2 |
| K2020054 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 3 |
| K2020055 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 4 |
| K2020056 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 5 |
| K2020057 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 6 |
| K2020058 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 7 |
| K2020059 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 8 |
| K2020060 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 9 |
| K2020061 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 10 |
Best New Basic Part
BBa_K2020042 - tRNA specific for tyrosine and UAG codon in E. coli
Best New Composite Part
BBa_K2020002 – expression system for subtilisin E in E. coli
This expression system consists of the promoter BBa_R0011, the ribosome binding site BBa_B0034, the newly created BioBrick part BBa_K2020001 and the terminator BBa_B0010. BioBrick BBa_K2020001 is a composite part itself and includes the secretion tag pelB (BBa_J32015) and a subtilisin E gene optimized for Escherichia coli codon usage (BBa_K2020000). Once introduced into E. coli, this BioBrick is able to produce subtilisin E, an alkaline serine protease which non-specifically digest proteins, and simultaneously secret the enzyme into the periplasm of the cell.
Caused of the lacI regulated promoter BBa_R0010, this expression system can be induced by addition of IPTG while working with LB medium.
Parts Collection
K2020043 – tRNA synthetase specific for the ncAA AzF and UAG codon in E. coli → AzF-RS
K2020047 – tRNA synthetase specific for the ncAA 5HTP and UAG codon in E. coli → 5HTP-RS
K2020050 - tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS
K2020051 - mutated tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS with Y32G
K2020052 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 1
K2020053 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 2
K2020054 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 3
K2020055 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 4
K2020056 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 5
K2020057 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 6
K2020058 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 7
K2020059 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 8
K2020060 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 9
K2020061 – tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 10
