Parts
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Weak promoter: Wanted a weak promoter to measure its PET plastic degradation up against the other promoters
Moderate promoter: Wanted a moderate promoter to measure its PET plastic degradation up against the other promoters
Strong promoter: Wanted a strong promoter to measure its PET plastic degradation up against the other promoters
N-Osmy tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest
C-Myc tag: Make our PETase construct easier to detect for Western Blot to assay expression and secretion
PETase sequence: Needed the enzyme to degrade PET plastic
Plasmid backbone: pSB1C3: Best vector to move our ligated DNA produced in Gibson Assembly
High efficiency e. Coli cells: Were the host for our optimized PETase
