Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Parts Existing parts used for the project In order to construct our own parts, we chose several existing parts from the registry to complete the coding sequence with promoters, RBS and terminators: Name Type Description Designer Length (bp) [http://parts.igem.org/Part:BBa_J23119/ BBa_J23119] Basic Constitutive promoter family member iGEM2006_Berkeley 35 [http://parts.igem.org/Part:BBa_B0030/ BBa_B0030] Basic RBS.1 (strong) - modified from R. Weiss Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003 15 [http://parts.igem.org/Part:BBa_B0015/ BBa_B0015] Basic Double terminator (B0010-B0012) (rrnB T1 - T7Te) Reshma Shetty, 2003 129 Parts designed for the project In order to achieve our project; we designed several basic and composite parts: Name Type Description Length (bp) [http://parts.igem.org/Part:BBa_K2039003/ BBa_K2039003] Basic Coding sequences of proteins FKBP and GFP10 528 [http://parts.igem.org/Part:BBa_K2039004/ BBa_K2039004] Basic Coding sequences of proteins FRB* and GFP11 498 [http://parts.igem.org/Part:BBa_K2039000/ BBa_K2039000] Composite Part expressing the dimerization protein FKBP and GFP10 731 [http://parts.igem.org/Part:BBa_K2039001/ BBa_K2039001] Composite Part expressing the dimerization protein FRB* and GFP11 701 [http://parts.igem.org/Part:BBa_K2039002/ BBa_K2039002] Composite Dimerization system FRB*/FKBP linked to two subunit of tripartite GFP (GFP 10 & GFP 11) 1440
In order to construct our own parts, we chose several existing parts from the registry to complete the coding sequence with promoters, RBS and terminators:
In order to achieve our project; we designed several basic and composite parts:
