Team:UST Beijing/Model
Model
β-galactosidase is used to deglycosylate saponin of notoginseng. Our Lab have a PET-28a plasmid withβ-galactosidase gene and LacI gene. The transcription of β-galactosidase is repressed by LacI protein. But lactose and IPTG can induce the expression of LacI protein. We used a 3L fermentation tank to conduct preliminary experiments, then the enzyme was extracted from bacteria solution using glycine buffer.
Enzyme activity
As Chinese traditional medicinal materials, notoginseng has been widely recognized on its efficacy by Chinese people during thousands of years. With the developing of modern medicine, other characteristics of notoginseng have been utilizing. Now it’s been proved that notoginsenoside has therapeutic effect on hyperlipidemia and cardiovascular diseases. However, the bioavailability of saponin of notoginseng in human body can reach only 4%. Based on this premise, we hope to hydrolyze glycosyl on saponin of notoginseng molecule out of body, so that deglycosylated saponin of notoginseng can be easy for human to absorb.
