Team:NYMU-Taipei/Notebook-Lab Book-C120 minPgpdA RFP TtrpC

C120_minPgpdA_RFP_TtrpC

C120_empty

minimal-PgpdA

RFP

TtrpC

C120_minPgpdA_RFP_TtrpC


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7/31

Transformed C120_minimal PgpdA into competent cells

C120_minimal PgpdA containing cells used in 2 in 1

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8/1

  • C120_minimal PgpdA extracted from transformed cells

  • C120_minimal PgpdA checked with restriction digestion

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    • 8/2

    C120_minimal PgpdA amplified with plasmid PCR

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    8/3

    C120_minimal PgpdA digested for ligase with RFP

    C120_minimal PgpdA_RFP transformed into competent cell

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    8/4

    C120_minimal PgpdA_RFP extracted from transformed cells

    C120_minimal PgpdA_RFP amplified with plasmid PCR and checked with restriction digestion

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    8/6

    C120_minimal PgpdA_RFP plasmid stored and transformed cultures cryopreserved

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    8/14

    C120_minimal PgpdA_RFP digested for ligation with TtrpC

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    8/15

    C120_minimal PgpdA_RFP_TtrpC transformed into competent cell

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    8/17

    C120_minimal PgpdA_RFP_TtrpC extracted and checked with restriction digestion

    C120_minimal PgpdA_RFP_TtrpC amplified with plasmid PCR

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    8/18

    C120_minimal PgpdA_RFP_TtrpC plasmid stored and transformed bacterial cultures cryopreserved

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