Thursday, August 11th
Tasks:
Paul
- Submitted sequencing of the 08.09 miniprep (w/ antibiotic) of Jordan’s 1:3 Gibson
- “1”= VF’
- “2”= Cas9_Seq1
- “3”= Cas9_Seq2
- “4”= Cas9_Seq3
-
- PCR of Tet lnrz for GFP (DpnI for 2 hr @ 37°C)
- Presentation for profs
Sam
- Provided Building with Biology to UNSW (and verified that you’re allowed to do that)
- Told them about our experiences so far
- Updated outreach log
- Improved Collaboration slide in presentation
- Updated Jamboree costs spreadsheet
- Additional Eligo editing
- Autoclaved Paul’s NaCl the normal way (and also some nearby diH2O just for kicks)
- Found out that autoclaving kills DNases but not RNases
Sara
- Made GG PCR parts fmol dilutions
Tyler
- Made Sequencing premix rxns for Jordan's 1:3 Cas9 08.09.16 miniprep
- 3.7 µL of 1:3 Jordan Cas9
- 1.2 µL of each primer (VF, Seq1, Seq2, Seq3)
- 10.1 µL of water
- RTW PCR of Cas9 part for insertion of signal sequences
- 2x 50 µL tubes (one with DMSO, one without)
- 21.5 µL nf water/22.5 µL nf water (tube w/o DMSO)
- 1 µL DMSO/0 µL DMSO
- 0.5 µL Cas9 Template (Jordan’s 1:3)
- 1 µL SS_Lnrz_FWD
- 1µL SS_Lnrz_RE
- 25 µL Taq Master Mix
- 1x50µL negative control
- 22 µL nf water
- 1 µL DMSO
- 1 µL SS_Lnrz_FWD
- 1 µL SS_Lnrz_REV
- 25 µL Taq Master Mix
- DpnI digest for 2 hours at 37°C (0.5 uL/tube)
- Presentation for profs
Paul
- Submitted sequencing of the 08.09 miniprep (w/ antibiotic) of Jordan’s 1:3 Gibson
- “1”= VF’
- “2”= Cas9_Seq1
- “3”= Cas9_Seq2
- “4”= Cas9_Seq3
- PCR of Tet lnrz for GFP (DpnI for 2 hr @ 37°C)
- Presentation for profs
Sam
- Provided Building with Biology to UNSW (and verified that you’re allowed to do that)
- Told them about our experiences so far
- Updated outreach log
- Improved Collaboration slide in presentation
- Updated Jamboree costs spreadsheet
- Additional Eligo editing
- Autoclaved Paul’s NaCl the normal way (and also some nearby diH2O just for kicks)
- Found out that autoclaving kills DNases but not RNases
Sara
- Made GG PCR parts fmol dilutions
Tyler
- Made Sequencing premix rxns for Jordan's 1:3 Cas9 08.09.16 miniprep
- 3.7 µL of 1:3 Jordan Cas9
- 1.2 µL of each primer (VF, Seq1, Seq2, Seq3)
- 10.1 µL of water
- RTW PCR of Cas9 part for insertion of signal sequences
- 2x 50 µL tubes (one with DMSO, one without)
- 21.5 µL nf water/22.5 µL nf water (tube w/o DMSO)
- 1 µL DMSO/0 µL DMSO
- 0.5 µL Cas9 Template (Jordan’s 1:3)
- 1 µL SS_Lnrz_FWD
- 1µL SS_Lnrz_RE
- 25 µL Taq Master Mix
- 1x50µL negative control
- 22 µL nf water
- 1 µL DMSO
- 1 µL SS_Lnrz_FWD
- 1 µL SS_Lnrz_REV
- 25 µL Taq Master Mix
- DpnI digest for 2 hours at 37°C (0.5 uL/tube)
- Presentation for profs
