Team:NYMU-Taipei/Notebook-Lab Book-pBAR pMCL1 KR TtrpC

pBAR_pMCL1_KR_TtrpC

pBARGPE1

PMCL1

BBa_K118400

TtrpC

pBAR_pMCL1_KR_TtrpC


[Collapse all] | [Expand all]

9/11

  • pBAR_KR transformed into competent cell

    • [Collapse]

    9/12

  • pBAR_KR performed plasmid extraction and plasmid PCR (successful)

    • [Collapse]

    9/14

  • K118400 and TtrpC used PCR to amplify the target sequence

  • KR_TtrpC used PCR to amplify the target sequence

    • [Collapse]

    9/15/div>

  • KR_TtrpC digested with restriction enzymes

    • [Collapse]

    9/16

  • pBAR_KR_TtrpC transformed into competent cell

  • Transformed pBAR_KR_TtrpC into competent cell

  • Digested pBAR_KR for transforming into Metarhizium anisopliae

    • [Collapse]

    9/17

  • pBAR_KR_TtrpC did 3 in 1

    • [Collapse]

    9/18

  • pBAR_KR_TtrpC did plasmid PCR and digestion check

  • Extracted plasmid pBAR_KR_TtrpC

    • [Collapse]

    9/20

  • KR_TtrpC digestion

  • Transformed pBAR_KR_TtrpC into competent cell

  • pBAR_KR_TtrpC did 3 in 1

  • Extracted plasmid pBAR_KR_TtrpC

  • pBAR_KR_TtrpC did digestion check

    • [Collapse]

    9/21

  • pBAR_KR_TtrpC did digestion check

    • [Collapse]

    9/22

  • Extracted plasmid pBAR_KR_TtrpC

  • pBAR_KR_TtrpC did plasmid PCR

  • pMCL1 and pBAR_KR_TtrpC digestion (pMCL1 was not digested successfully)

    • [Collapse]

    9/25

  • pMCL1 and pBAR_KR_TtrpC digestion

  • Transformed pBAR_pMCL1_KR_TtrpC into competent cell

  • pBAR_pMCL1_KR_TtrpC did 3 in 1

    • [Collapse]

    9/26

  • Extracted plasmid pBAR_pMCL1_KR_TtrpC

    • [Collapse]

    9/28

  • pBAR_pMCL1_KR_TtrpC did digestion check

    • [Collapse]

    9/30

  • pBAR_pMCL1_KR_TtrpC did digestion check

    • [Collapse]