Thursday, August 18th
Tasks:
Jordan
- Ran RTW PCR of Cas9 for SS with Sara
- Inoculated overnight cultures of JC8031 and JC8031-pClyA-GFP-His6
- Gel extracted linearized Cas9 for signal sequences
Michelle
- Ran gel for linearized Cas9 and linearized mRFP
- Ran gel for 8/16/16 gibson reaction products
- 1: 2kb Ladder
- 3: Cas9 Gibson with TorA signal (expected size: ~5kb; parts: ~5k, ~300bp)
- 5: Cas9 Gibson with INP fusion (expected size: ~6kb; parts: ~5kb, ~1kb)
- 7: mRFP Gibson with gRNA (expected size: ~4kb; parts: ~3.5kb, 400bp)
- 9: Gibson kit positive control
- 10: No Gibson mix negative control w/ Cas9 and TorA (expected sizes: ~5kb, ~300bp)
-
Paul
- Worked on presentation for profs
- Helped set up GFP/mCherry PCR (Thush did it)
- Troubleshoot Gibson with Kelly
Sam
- Finally put research in the research doc
- Looked over presentation for tomorrow
- Started designing antibiotics forum
Jordan
- Ran RTW PCR of Cas9 for SS with Sara
- Inoculated overnight cultures of JC8031 and JC8031-pClyA-GFP-His6
- Gel extracted linearized Cas9 for signal sequences
Michelle
- Ran gel for linearized Cas9 and linearized mRFP
- Ran gel for 8/16/16 gibson reaction products
- 1: 2kb Ladder
- 3: Cas9 Gibson with TorA signal (expected size: ~5kb; parts: ~5k, ~300bp)
- 5: Cas9 Gibson with INP fusion (expected size: ~6kb; parts: ~5kb, ~1kb)
- 7: mRFP Gibson with gRNA (expected size: ~4kb; parts: ~3.5kb, 400bp)
- 9: Gibson kit positive control
- 10: No Gibson mix negative control w/ Cas9 and TorA (expected sizes: ~5kb, ~300bp)
Paul
- Worked on presentation for profs
- Helped set up GFP/mCherry PCR (Thush did it)
- Troubleshoot Gibson with Kelly
Sam
- Finally put research in the research doc
- Looked over presentation for tomorrow
- Started designing antibiotics forum
