Team:NYMU-Taipei/Notebook-Lab Book-pGFP C120 RFP

pGFP_C120_RFP

pBARGPE1

GFP

TtrpC

C120_empty

minimal-PgpdA

RFP

pGFP_C120_RFP


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9/23

  • GFP_TtrpC ligation PCR

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    9/24

  • GFP_TtrpC ligation PCR

  • GFP_TtrpC ligation PCR (We confirmed junction primer was wrong)

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    9/30

  • Received the junction primer of GFP-TtrpC

  • GFP_TtrpC ran ligation PCR

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    10/1

  • GFP_TtrpC ran ligation PCR

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    10/2

  • GFP_TtrpC digested for ligation with pBARGPE1

  • Transformed pBAR_GFP_TtrpC into competent cells

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    10/3

  • pBAR_GFP_TtrpC containing cultures used in 2 in 1

  • pExtracted plasmid pBAR_GFP_TtrpC

  • pBAR_GFP_TtrpC digestion check(failure)

  • pBAR_GFP_TtrpC PCR check(failure)

  • Transformed pBAR_GFP_TtrpC into competent cells

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    10/4

  • pBAR_GFP_TtrpC containing cultures used in 2 in 1

  • pBAR_GFP_TtrpC plasmid extraction

  • pBAR_GFP_TtrpC digestion check

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