Team:NYMU-Taipei/Notebook-Lab Book-pBARGPE1

pBARGPE1

pBARGPE1


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6/29

  • Received plasmid pBARGPE1 From Bioresource Collection and Research Center

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    7/11

  • pBARGPE1 resuspended (from cryovial)

  • Transformed pBARGPE1 into competent cells

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    7/12

  • pBARGPE1 containing cultures used in 2 in 1

  • pBARGPE1 extracted from transformed cultures

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    7/13

  • pBARGPE1 checked with restriction digestion (EcoRI和SpeI)

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    7/21

  • pBARGPE1 plasmid stored and transformed cultures cryopreserved

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    7/26

  • pBARGPE1 digested for pBAR_RFP construction

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    7/30

  • pBARGPE1 digested (concentration not high enough)

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    8/1

  • pBARGPE1 digested for pBAR_RFP construction

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    8/4

  • pBARGPE1 digested for pBAR_EL222_TtrpC construction

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    8/5

  • pBARGPE1 digested for pBAR_PgpdA construction

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    8/15

  • pBARGPE1 digested for pBAR_mRFP1_TtrpC construction

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    8/16

  • pBARGPE1 digested for pBAR_EL222_TtrpC construction

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    8/27

  • pBARGPE1 checked with restriction digestion due multiple failed attempts to ligate pBARGPE1 with various inserts

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    9/2

  • pBARGPE1 digested for pBAR_EL222_TtrpC construction

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    9/9

  • pBARGPE1 digested for pBAR_mRFP1_TtrpC construction

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    9/10

  • pBARGPE1 digested for pBAR_PgpdA_EL222_TtrpC construction

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    9/11

  • pBARGPE1 digested for pBAR_PgpdA_EL222_TtrpC construction

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    9/15

  • pBARGPE1 digested for pBAR_KR_TtrpC construction

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    10/2

  • pBARGPE1 digested for pBAR_GFP_TtrpC construction

  • pBARGPE1 resuspended (from cryopreservation)

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    10/3

  • Extracted plasmid pBARGPE1

  • pBARGPE1 checked with restriction digestion (correct)

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    10/4

  • pBARGPE1 plasmid stored

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