Team:Northeastern/Basic Part

basic parts

NADH Oxidase

This is a novel part submitted to the registry from the NCBI database, NADH oxidase/NADH dehydrogenase from Streptococcus sanguinis. Nox encodes the H2O-forming NADH oxidase enzyme that catalyses the oxidation of NADH to form water:

4 NADH + O₂ → 4 NAD⁺ + 2 H₂O + 4 e^-

We also designed a Nox protein with an N-terminus His tag to purify the protein using ELISA (BBa_K2182001), and modified RBS sequences based on the one submitted with the fnr coding sequence by Paris Saclay 2013 optimized to reduce homodimer formation during PCR (BBa_K2182004 and BBa_K2182005).

Summary Composite Collection

Northeastern University, Boston MA · Github