Team:CSU Fort Collins/NoteBook/JanThroughApr
January Through April
1/4/16:
Ligated b0034 and c0061
Transformed Ligated mixture
Ran PCR results on a 2% gel
1/5/16:
Digested LuxR with EcoRI and SpeI
Performed Colony PCR on transformation from the 4th
1/11/16:
slr0435 Excision from Synechocystis sp. PCC6803 with PCR using Phusion(size 276 bp).
Ran PCR results on a 2% gel
1/12/16:
Digested slr0435 and resuspended pSB1C3 with EcoRI and PstI
Ligated pSB1C3 and slr0435
Transformed ligation mixture
1/13/16:
Colony PCR performed on slr0435+pSB1C3 transformed colonies
Ran PCR results on 1% gel
2/6/16:
PCRed out BBA_J23106 out of glycerol stock 19 using Q5 master mix 19rxns
2/9/16:
Transformed Bba_K325909 from parts kit into DH5a cels
2/11/16:
To confirm that Bba_K325909 are functioning we tried to induce expression using L-arabinose
No luminescence was produced
3/24/16:
Set up O/N cultures of K1033929, K592009 and E1010 in 5 mL of LB with 5 uL of Cm34 antibiotic
3/29/16:
Ran promoters Sll027, and LrtA in a 1% gel
Sll0027
LrtA
4/6/16:
Ran Cpcg2 on 1% gel
