Team:Northwestern/sequencing

Protocols

Submitting Sequencing Reactions

  • Use small 600 uL tubes
    • 1 template+1 primer per tube
    • 450-600 ng of DNA template (~100-800)
    • 1.2 uL of 10 uM primer
    • Water to 15 uL
  • Submit sequencing through Genesifter account (Paul)
  • Deliver to Hogan 2-100 before 10:00am for pick up