Team:CSU Fort Collins/NoteBook/Aug

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CSU iGEM



August

8/5/16:
  • Phosphorylated Qblocks and pSB1C3
  • Performed ligase cycling on phosphorylated mixture, but instead used an annealing temperature of 55C
  • Gel had no bands
8/6/16:
  • Performed Colony PCR on Slr0118+Luc transformed colonies
  • Ran PCR results on a 1% gel
  • Gel had no bands
8/8/16:
  • Digested I712013 and Luc
  • Ligated I712013 and Luc
  • Transformed ligation mixture
8/9/16:
  • PCRed out T7 polymerase N and C from T7 polymerase
8/11/16:
  • Colony PCRed transformed 21 I7+Luc colonies
8/12/16:
  • Phosphorylated Q1 and Q2 for ligase cycling
8/15/16:
  • Performed ligase cycling reaction on phosphorylated Q block mixture
8/19/16:
  • Performed colony PCR on Slr0168+Luc
8/23/16:
  • Performed colony PCR on Slr0168+Luc and Quorum block
  • Transformed Slr0168+Luc ligation mixture
  • Performed 2-step PCR on Q1 and Q2
  • RePCRed Q1,Q2,and pSB1C3 for ligase cycling
8/25/16:
  • Digested Slr0168(ECORI PstI) and Luc(ECORI PstI)
  • Ligated Slr0168 and Luc overnight at 16C
8/26/16:
  • Slr0168+Luc and Qblock transformed colonies were colony PCRed with Q5 master mix
  • Ran PCR products from the 23rd on a 1.5% gel