Team:Aix-Marseille/Notebook WIP

Monday, June 13th

• Preparation of heat competent DH5α cells

Tuesday, June 14th

• Transformation of DH5α cells with test plasmid

Wednesday, June 15th

• Preparation of heat competent Tg1 cells
• Transformation of Tg1 cells with test plasmid

Thursday, June 16th

• Transformation of DH5α cells with pSB1C3 + RFP/li>
• Transformation with K880005, K823017, I0500, K1404006, B0034

Friday, June 17th

• Miniprep K880005, K823017, I0500, K1404006, B0034
• Miniprep pSB1C3 + RFP

Monday, June 20th

• pSB1C3 digestion from 17/06 miniprep
• pSB1C3 digestion gel migration : NO PHOTO
• Biobricks minipreps gel migration : OK PHOTO
• Transformation Tg1 with RFP+pSB1C3

Tuesday, June 21th

• pSB1C3 digestion and gel migration, NucleoSpin gel purification and PCR cleanup
• FliC E.Coli and FliC Desulfo digestion E/P and ligation with pSB1C3
• E. Coli and Desulfo FliC ligation in linearized pSB1C3
• Transformation of pSB1C3 RFP in Tg1

Wednesday, June 22th

• Transformation in DH5α of :
  • FliC coli/desulfo in pSB1C3
  • Linearized pSB1K3
  • Linearized pSB1A3
  • Linearized pSB1T3
  • Linearized pSB1C3
• Miniprep B0034
• Transformation in DH5α of interlab studies plasmids